Method for evaluating genetic relationship DNA (deoxyribonucleic acid) molecules of mulberry variety

Abstract

The invention discloses a method for evaluating genetic relationship DNA (deoxyribonucleic acid) molecules of a mulberry variety. The method comprises the steps of (1) system development analysis of the mulberry variety: 1) material selection; 2) screening of a contrast sequence; 3) extraction of mulberry genome DNA and detection of purity and concentration; 4) PCR (polymerase chain reaction) amplification; 5) PCR detection; 6) PCR sequencing; 7) sequence splicing and comparison, and identification of an evolutionary relationship; (2) genetic relationship analysis of the mulberry variety. According to the method disclosed by the invention, the system development analysis is firstly performed, materials are reasonably selected from worldwide mulberry varieties and variants, and then genetic diversity analysis of mulberry variety populations is performed to further determine the genetic relationship, so that the accuracy and repeatability of evaluating the genetic relationship are significantly improved by using the two-step analysis method, and a great promotion effect is achieved for protection of mulberry variety resources, mulberry variety fingerprint identification, molecular aided breeding and variety resource development and utilization.

Description

technical field [0001] The invention belongs to the technical field of mulberry variety breeding and molecular biology, and in particular relates to a DNA molecular evaluation method for genetic relationship of mulberry varieties. Background technique [0002] my country is a big silk producing country and the birthplace of the world's sericulture. Sericulture production occupies a very important position in the national economy. Mulberry is an important feed for silkworms, and the development and breeding of mulberry variety resources has become an increasingly important research topic. At present, many scholars at home and abroad have already begun to preserve mulberry variety resources in theory and practice, the selection of hybrid breeding parents, Conduct research on fingerprint identification of mulberry varieties, molecular assisted breeding, development and utilization of variety resources. [0003] So far, a number of classic taxonomic studies have been carried ou...

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Problems solved by technology

[0005] The purpose of the present invention is to overcome the poor repeatability of the experimental results of the evaluation method in the prior art, which is not suitable for the phylogenetic analysis of mulberry varieties, the defects such as the inability to subdivide the Morus varieties, poor accuracy, unreasonable reference value of materials, etc., to provide A DNA molecular evaluation method for genetic relationship of mulberry species. This method is simple and easy to use, and the materials are reasonable. It can determine the general framework of genetic relationship through phylogenetic analysis, and then further analyze the genetic diversity of populations based on the framework, thereby improving the genetic relationship. Accuracy of Relationship Identification

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