Preparation and application of CS/IL-GR modified bovine serum albumin molecular imprinting electrode

Abstract

The invention discloses a preparation of a CS / IL-GR modified bovine serum albumin molecular imprinting electrode. The preparation comprises the following steps of the preparation of a CS / IL-GR / GCE, and the preparation of MIPs / CS / IL-GR / GCE. The invention also discloses an application of the CS / IL-GR modified bovine serum albumin molecular imprinting electrode to the detection on BSA. The application is characterized in that a [Fe(CN)6]<3- / 4-> is adopted as an electrochemical probe, an electrochemical impedance (EIS) and a differential pulse voltammetry (DPV) are adopted to represent a property of the MIPs / CS / IL-GR / GCE, the imprinting electrode can express a good linear relation on the BSA within a 1.0*10<-10>-1.0*10<-4>g / L concentration range, the peak current change value deltaI can be calculated as follows: deltaI (muA)=27.42+2.21*log(CBSA)(g / L), R=0.996, and the detection limit can be up to 2.02*10<-11>g / L. The preparation method provided by the invention has the beneficial effects that an imprinting sensor can express the good selectivity, sensitivity and reproducibility based on a synergistic effect of an IL-GR compound and the interaction of a chitosan molecule and a protein molecule, and the preparation method is used for clinical analysis detection on a trace sample.

Description

technical field [0001] The invention relates to an electrochemical sensor, in particular to the preparation and application of a bovine serum albumin molecularly imprinted electrode modified by CS / IL-GR. Background technique [0002] Molecularly imprinted electrochemical sensors (MIECS) combine electrochemical sensors and molecularly imprinted technologies, using molecularly imprinted polymers as biorecognition elements to improve the sensitivity and selectivity of electrochemical sensors, and have been successfully used to imprint small biological molecules. At the same time, imprinting biomacromolecules, especially imprinted protein molecules, and applying them to electrochemical detection has attracted more and more attention of researchers. However, the sensitivity of electrochemical sensors or biosensors with imprinted protein molecules is limited by the large size, variable structure and low rebonding efficiency of protein molecules, and the research progress is slow. ...

AI Technical Summary

Problems solved by technology

However, the sensitivity of electrochemical sensors or biosensors of imprinted protein molecules is limited by the large size of protein molecules, variable structure and low rebonding efficiency, and the research progress is slow.

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