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Method for measuring substances

A determination method and technology for substances, which are applied in the determination/inspection of microorganisms, biochemical equipment and methods, and measuring devices, etc., can solve problems such as removing electrochemical reactions, and achieve suppression of concentration changes, stable oxidation current, and improved measurement accuracy. Effect

Inactive Publication Date: 2014-07-23
MURATA MFG CO LTD
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Problems solved by technology

[0010] In the above-mentioned conventional method for measuring substances, since the adjustment potential with a potential lower than the measurement potential is applied to the working electrode, it cannot be removed from the working electrode and the counter electrode when the measurement potential with a potential higher than the adjustment potential is applied to the working electrode. Impurities that carry out electrochemical reactions, thus requiring improved technology

Method used

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  • Method for measuring substances
  • Method for measuring substances

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Embodiment Construction

[0038] refer to Figure 1 to Figure 8 , one embodiment of the method for measuring a substance of the present invention will be described.

[0039] figure 1 It is a figure which shows an example of the biosensor system used for the measuring method of the substance of this invention. figure 2 It is a figure which shows an example of a biosensor, (a) is an exploded perspective view, (b) is a perspective view. image 3 It is a flowchart showing an example of measurement processing. Figure 4 It is a graph showing an example of the potential applied to the working electrode based on the counter electrode. Figure 5 It is a graph showing an example of a response current obtained by applying a measurement potential to a working electrode. Figure 6 It is a graph showing a comparative example of a response current obtained by applying a measurement potential to a working electrode. Figure 7 It is a graph showing the relationship between the magnitude of the adjustment potent...

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Abstract

Provided is a technique that enables measurement precision to be improved when quantifying a substance to be measured, which is contained in a sample, by decreasing the impact of current components different from the oxidation current resulting from the oxidization of a reducing substance produced by a reaction between enzymes and the substance to be measured in the sample, among the current components in a response current obtained by applying a counter electrode-based measurement potential to a working electrode. Because an adjustment potential with higher potential than a measurement potential is applied in the form of a pulse to the working electrode, among the current components contained in the response current obtained by applying the counter electrode-based measurement potential to the working electrode, the impact of the current components different from the oxidation current resulting from oxidization of the reducing substance produced by the reaction between the substance to be measured and enzymes in the sample can be reduced. Thus, the response current can be stably measured, and measurement precision can be improved when quantifying the substance to be measured, which is contained in the sample.

Description

technical field [0001] The present invention relates to a substance measurement method for quantifying a measurement target substance contained in a subject using a biosensor. Background technique [0002] Conventionally, there is known a method for measuring a substance in which the substance to be measured is quantified by measuring an oxidation current using a biosensor having a chamber for supplying a subject, a containment function, An electrode system of an electrode and a counter electrode, and a reaction layer including an enzyme that specifically reacts with a substance to be measured, and the oxidation current is obtained by applying a measurement potential based on the counter electrode to the working electrode to oxidize the reducing substance The reducing substance is a substance generated by the reaction between the measurement target substance contained in the subject supplied to the chamber and the reaction layer. [0003] The biosensor consists of stacking ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/416G01N27/327
CPCG01N27/3273C12Q1/001C12Q1/006
Inventor 大江秀明高木纯横山宪二平塚淳典吉田信行佐佐木典子
Owner MURATA MFG CO LTD
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