Sugarcane callus coloring controlling gene ScSN and application thereof

A technology of callus and color control, applied in application, genetic engineering, plant genetic improvement, etc., to achieve high food safety, eliminate ecological risks, and good color rendering effect

Inactive Publication Date: 2014-08-06
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently widely used marker genes such as GUS and GFP have certain deficiencies and defects, for example, they have no practical application value except for reporting function; The protein needs to consume plant assimilates, but it is not beneficial to plant growth, etc., and the anthocyanin transcription factor gene can not only play the function of marker gene, but also plays an important role in the growth of plants. Therefore, the expression of anthocyanin related genes Research is particularly important

Method used

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  • Sugarcane callus coloring controlling gene ScSN and application thereof
  • Sugarcane callus coloring controlling gene ScSN and application thereof
  • Sugarcane callus coloring controlling gene ScSN and application thereof

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Embodiment 1

[0026] A sugarcane callus color control gene ScSN and applications thereof, specifically including sugarcane anthocyanin-regulated genes ScSN Cloning and genetic transformation of , as follows:

[0027] (1) PCR primer design: Inquire about corn on NCBI SN (GenBank: X60706.1) sequence, primers were designed at the start codon and stop codon positions according to the sequence, the sequence is:

[0028] SNF1: 5'-ATGGGCCTTCAGCTTCCCGAGTTC-3';

[0029] SNR1: 5'-TCACCGCTTCCCTATAGCTTTGCGA-3'.

[0030] (2) Extraction of total RNA from sugarcane leaves

[0031] ① Take fresh sugarcane leaves, wipe the surface of the leaves with 75% alcohol, and quickly grind them into powder under the action of liquid nitrogen. Transfer the ground material to a 1.5 mL Eppendorf centrifuge tube pre-added with 1 mL of TRIZOL reagent, shake vigorously, mix well, place at room temperature for 5 min, and centrifuge at 4 °C and 12 000 rpm for 5 min.

[0032]②Carefully draw 800 μl of the supernata...

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Abstract

The invention provides a sugarcane callus coloring controlling gene ScSN and an application thereof. A primer is designed according to upstream and downstream sequences of a maize anthocyanin transcription factor gene SN (GenBank:X60706.1), and a complete sequence of the anthocyanin transcription factor gene ScSN is obtained from sugarcane by utilizing homology-based cloning; the length of an open reading frame of a sugarcane ScSN gene is 1722bp, and 573 amino acids are encoded; BLAST analysis is carried out on an encoding region sequence of the sugarcane ScSN gene in NCBI (National Center For Biotechnology Information), and an E value is equal to zero in BLAST homology search of numerous genes relative to the maize anthocyanin, showed that the cloned gene is a relative gene synthesized by the sugarcane anthocyanin really; a Kpn I restriction site and a Sal I restriction site are added at the two ends of the ScSN sequence respectively, a plant expression vector pCAMBIA1301-35SN-ScSN is constructed, sugarcane callus is transformed by gene gun bombardment, the color of the transformed sugarcane callus is converted to kermesinus, and further verified that the cloned gene has the function of enabling the sugarcane callus to carry out coloration.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a sugarcane callus color development control gene ScSN and an application thereof. Background technique [0002] Anthocyanins, also known as anthocyanins and anthocyanins, are a class of plant-derived water-soluble pigments that are ubiquitous in nature. Anthocyanins are also the main factor for the coloration of plants, and the different pH values ​​in the plant vacuoles are the key to affecting the different colors of plant flowers. Anthocyanins in nature can not only make plants present rich and colorful colors, which is beneficial to plant pollination, but also protect plant fruits from ultraviolet rays. Previous studies have shown that anthocyanins have strong antioxidant capacity, which is 20 times higher than vitamin C and 50 times higher than vitamin E. It is an effective scavenger of free radicals, and has high nutritional and health functions in the preventi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C07K14/415A01H5/00
Inventor 陈平华施桂姣王恒波张卓陈忠伟高三基项慰刘迪邰连赛林冰陈利平许莉萍陈如凯
Owner FUJIAN AGRI & FORESTRY UNIV
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