Reticulitermes-flavipes indoor intensive breeding method

A termite and nest technology, applied in animal husbandry and other directions, can solve the problems of difficulty in standardizing and unifying, increasing the chance of termite infection, and inability to artificially large-scale intensive feeding, and achieve the effect of simple operation and high success rate.

Active Publication Date: 2014-09-10
HUAZHONG AGRI UNIV
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These cultivation methods have the following defects: (1) the bait and the substrate are mixed together, which increases the chance of termite infection; (2) the substrate and the bait source are natural primary products, which are not easy to standardize and unify, and cannot carry out artificial large-scale intensive breeding

Method used

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  • Reticulitermes-flavipes indoor intensive breeding method

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Embodiment Construction

[0014] The invention consists of key steps such as selection of nesting container, preparation of culture substrate, preparation of artificial bait, preparation of nest room for cultivation, acquisition of nesting dewing ants, regular inspection and maintenance, anatomical observation and the like. Specific steps are as follows:

[0015] 1) Use an optically transparent pure polystyrene 6-well cell culture plate as the nest container, and add 3-5mL of sterile water to make the inner pad volume of each well 1 is 7mL and pure natural aseptic vermiculite 3 with a diameter of 1-2mm. The sterile vermiculite is completely soaked, but there is no water; after sterilizing the oak wood powder that has undergone three deep fermentations, you can also use masson pine wood powder, sweetgum wood powder, white magnolia wood powder, and corn that have undergone three deep fermentations Rod powder and bagasse powder are used as bait 2, and an inclined plane is constructed on sterile vermiculit...

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Abstract

The invention relates to a reticulitermes-flavipes indoor intensive breeding method. Sterile vermiculite is placed in holes of a six-hole cell culture plate in a cushioning mode and drenched, and bait which is deep fermented three times is used for building a slope on the vermiculite and is compacted. Before reticulitermes flavipes swarms, withered stumps containing winged-reticulitermes-flavipes nests are fetched back, placed in storage boxes, and kept in a 16-DEG-C indoor dark condition until the reticulitermes flavipes grows and develops to be in a swarming state; swarming is facilitated by people, the reticulitermes flavipes is collected through a nylon net, and the male reticulitermes flavipes and the female reticulitermes flavipes are identified; a pair of reticulitermes flavipes paired successfully is placed in the hole with the corresponding number, and a plate cover is placed on the corresponding hole. The situations that the reticulitermes flavipes with wings falling off gets into soil and builds nests are observed until all the reticulitermes flavipes with the wings falling off gets into the soil and builds the nests, water is added to the vermiculite, the culture plate is placed in a culture room, and breeding is carried out for 7.5 months. By the adoption of the reticulitermes-flavipes indoor intensive breeding method, the nest-building success rate can reach 95%, 7-17 worker ants are bred in each nest, a soldier ant appears in 62.5% of the nests, and the nests with complete functions are formed; operation is simple, high-density intensive breeding can be carried out, the success rate is high, and resources are provided for research, prevention and treatment of the reticulitermes flavipes.

Description

technical field [0001] The invention belongs to the technical field of insect breeding, and in particular relates to a method for artificially cultivating scattered termite indoor nest clusters. Background technique [0002] Reticulitermes belong to Isoptera Rhinotermitidae, belong to lower termites, and are important pests that seriously harm houses, buildings and garden trees in the northern provinces of my country and the Yangtze River Basin. The scattered termite colony has higher requirements on the humidity of the environment, but likes humidity and is afraid of water. Whether it is a newly established colony or a developed colony, they all choose to build their nests in or near damp wood. This shows that they mainly obtain water from the food and the surrounding environment through the body wall, unlike higher termites, such as Coptotermes formosanus, which can obtain water by building water-absorbing lines. This has greatly increased the difficulty of indoor cultiva...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033
Inventor 黄求应李刚华雷朝亮
Owner HUAZHONG AGRI UNIV
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