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Modified corn steep liquor for fermentation production of beta-carotene, and preparation method and application thereof

A technology of carotene and corn steep liquor, which is applied in the field of bioengineering, can solve the problems of low added value, insufficient value, small dosage, etc., and achieve high yield effect

Active Publication Date: 2014-09-17
HUBEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But this is only used for the production of some extensive and low-value-added fermented products, the amount of which is small, and the value has not been fully reflected

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment example 1

[0023] Implementation Case 1: Pretreatment of Corn Steep Steep

[0024] Corn steep liquor was purchased from Shandong Weifang Shengtai Pharmaceutical Co., Ltd., and the production date was June 23, 2013.

[0025] 1. Use high performance liquid chromatography (HPLC), atomic absorption spectroscopy and other equipment to detect the content of each component in corn steep liquor. The results are shown in Table 1.

[0026] Table 1 Contents of each component in corn steep liquor

[0027] components Content (mg / kg) Ca 440 Cu 0.18 Mg 3500 Zn 61 Fe 396 mn 28.8 K 15200 glucose 13.2 maltose 2.182 reducing sugar 7.55 total sugar 17.32 organophosphate 9.261 Inorganic phosphorus 4.553 Total Amino Acids 101236 total nitrogen 42.715g / L

[0028] 2. Add 0.5% (W / V) Na 2 S process the corn steep liquor, use a mixer to make it fully react.

[0029] 3. Centrifuge the obtained corn steep liq...

Embodiment example 2

[0033] Implementation Case 2: Production and Preparation of β-Carotene Using Untreated Corn Steep Steep in Shake Flasks

[0034] 1. Strains:

[0035] Trispora boulardii ATCC 14271 (+) and ATCC 14272 (-)

[0036] 2. Preparation of medium:

[0037] Seed medium (W / V): corn flour 3%, soybean flour 3%, KH 2 PO 4 0.1%, MgSO 4 0.02%, V B1 0.001%, pH 6.5.

[0038] Fermentation medium formula (W / V): untreated initial corn steep liquor 8% (V / V), glucose 6%, MgSO 4 0.02%, KH 2 PO 4 0.2%, soybean oil 1% (V / V), V B1 0.001%, adjust pH6.5.

[0039] 3. Seed culture

[0040] Inoculate Mycobacterium trispora (+) (-) into the seed medium in the form of spore suspension, the seed medium filling volume is 50ml / 250mL Erlenmeyer flask, the culture temperature is 28°C, and the rotation speed is 200 rpm , the positive bacteria culture time is 20 hours, the negative bacteria culture time is 25 hours, and the positive and negative strain seed liquid of B. trispora is obtai...

Embodiment example 3

[0049] Implementation Case 3: Production of β-carotene by pretreated corn steep liquor in shake flasks

[0050] 1. Strains:

[0051] Trispora boulardii ATCC 14271 (+) and ATCC 14272 (-)

[0052] 2. Preparation of medium:

[0053] Seed medium (W / V): corn flour 3%, soybean flour 3%, KH 2 PO 4 0.1%, MgSO 4 0.02%, V B1 0.001%, pH 6.5.

[0054] Formula of fermentation medium (W / V): 8% (V / V) of improved corn steep liquor prepared in Example 1, 6% glucose, MgSO 4 0.02%, KH 2 PO 4 0.2%, soybean oil 1% (V / V), V B1 0.001%, adjust pH6.5.

[0055] 3. Seed culture

[0056] Inoculate Mycobacterium trispora (+) (-) into the seed medium in the form of spore suspension, the seed medium filling volume is 50mL / 250mL Erlenmeyer flask, the culture temperature is 28°C, and the rotation speed is 200 rpm , the positive bacteria culture time is 20 hours, the negative bacteria culture time is 25 hours, and the positive and negative strain seed liquid of B. trispora is obtaine...

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PUM

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Abstract

The invention discloses a modified corn steep liquor for fermentation production of beta-carotene, and a preparation method and an application thereof. The modified corn steep liquor is obtained by the steps of mixing a corn steep liquor raw material and Na2S, then carrying out a full reaction, centrifuging, and adjusting the pH of the supernatant to 6.3-6.7. Through physical and chemical pretreatment of the corn steep liquor, the raw material which can be fully used by blakeslea trispora positive bacteria ATCC14271 (+) and blakeslea trispora negative bacteria ATCC14272 (-) are obtained finally; and the modified corn steep liquor is used as raw material for fermentation, the higher yield of beta-carotene can be obtained, and the content of beta-carotene in dry mycelia is 2%-6% (W / W).

Description

technical field [0001] The invention belongs to the technical field of bioengineering for preparing beta-carotene by utilizing agricultural and sideline resources, and specifically relates to an improved corn steep liquor for fermenting and producing beta-carotene, a preparation method and application thereof. [0002] Background technique [0003] β-carotene (β-carotene) is a kind of carotenoid, which is recognized as a non-toxic, safe and nutritious food fortification by the Food and Agriculture Organization of the United Nations (AFO) and the World Health Organization (WHO). agent. In addition, as an antioxidant, β-carotene has good antioxidant properties, and has obvious functions in the prevention and treatment of many degenerative diseases caused by aging, such as cardiovascular diseases, cataracts and anti-cancer. Furthermore, β-carotene is also used as a food additive. Because it is a fat-soluble compound and its color difference at different concentrations can...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P23/00C12R1/645
Inventor 蔡俊向梦雄王常高周安盛
Owner HUBEI UNIV OF TECH