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A kind of improved corn steep liquor used for fermentation production of β-carotene and its preparation method and application

A carotene and corn steep liquor technology, applied in the field of bioengineering, can solve the problems of low added value, insufficient value, small dosage, etc., and achieve the effect of high yield

Active Publication Date: 2016-08-03
HUBEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But this is only used for the production of some extensive and low-value-added fermented products, the amount of which is small, and the value has not been fully reflected

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment example 1

[0021] Implementation Case 1: Pretreatment of Corn Steep Steep

[0022] Corn steep liquor was purchased from Shandong Weifang Shengtai Pharmaceutical Co., Ltd., and the production date was June 23, 2013.

[0023] 1. Use high performance liquid chromatography (HPLC), atomic absorption spectroscopy and other equipment to detect the content of each component in corn steep liquor. The results are shown in Table 1.

[0024] Content of each component in table 1 corn steep liquor

[0025] components

Content (mg / kg)

Ca

440

Cu

0.18

Mg

3500

Zn

61

Fe

396

mn

28.8

K

15200

glucose

13.2

3 --> Maltose

2.182

reducing sugar

7.55

total sugar

17.32

organophosphate

9.261

Inorganic phosphorus

4.553

Total Amino Acids

101236

total nitrogen

42.715g / L

[0026] 2. Add 0.5% (W / V) Na 2 S process the corn steep liquor, use a...

Embodiment example 2

[0030] Implementation Case 2: Production and Preparation of β-Carotene Using Untreated Corn Steep Steep in Shake Flasks

[0031] 1. Strains:

[0032] Trispora boulardii ATCC14271 (+) and ATCC14272 (-)

[0033] 2. Preparation of medium:

[0034] Seed medium (W / V): corn flour 3%, soybean flour 3%, KH 2 PO 4 0.1%, MgSO 4 0.02%, V B1 0.001%, pH6.5.

[0035] Fermentation medium formula (W / V): untreated initial corn steep liquor 8% (V / V), glucose 6%, MgSO 4 0.02%, KH 2 PO 4 0.2%, soybean oil 1% (V / V), V B1 0.001%, adjust pH6.5.

[0036] 3. Seed culture

[0037] Inoculate Mycobacterium trispora (+) (-) into the seed medium in the form of spore suspension, the seed medium filling volume is 50ml / 250mL triangular flask, the culture temperature is 28°C, the rotation speed is 200rpm, The culture time of positive bacteria is 20 hours, and the culture time of negative bacteria is 25 hours, and the seed liquid of positive and negative strains of B. trispora is obtained.

[0038] 4...

Embodiment example 3

[0045] Implementation Case 3: Production of β-carotene by pretreated corn steep liquor in shake flasks

[0046] 1. Strains:

[0047] Trispora boulardii ATCC14271 (+) and ATCC14272 (-)

[0048] 2. Preparation of medium:

[0049] Seed medium (W / V): corn flour 3%, soybean flour 3%, KH 2 PO 4 0.1%, MgSO 4 0.02%, V B1 0.001%, pH6.5.

[0050] Formula of fermentation medium (W / V): 8% (V / V) of improved corn steep liquor prepared in Example 1, 6% glucose, MgSO 4 0.02%, KH 2 PO 4 0.2%, soybean oil 1% (V / V), V B1 0.001%, adjust pH6.5.

[0051] 3. Seed culture

[0052] Inoculate Mycobacterium trispora (+) (-) into the seed medium in the form of spore suspension respectively, the seed medium filling volume is 50mL / 250mL Erlenmeyer flask, the culture temperature is 28°C, the rotation speed is 200rpm, The culture time of positive bacteria is 20 hours, and the culture time of negative bacteria is 25 hours, and the seed liquid of positive and negative strains of B. trispora is obta...

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PUM

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Abstract

The invention discloses an improved corn steep liquor for fermenting and producing β-carotene, a preparation method and application thereof. The improved corn steep liquor is the raw material of corn steep liquor and Na 2 S mixed and centrifuged after fully reacting, and the supernatant was adjusted to pH=6.3‑6.7. The present invention pretreats corn steep liquor by physical and chemical means, and finally obtains raw materials that can be fully utilized by B. trispora positive bacteria ATCC 14271 (+) and B. trispora negative bacteria ATCC 14272 (‑). Higher yield of β-carotene can be obtained by fermenting the improved corn steep liquor as raw material, and the content of β-carotene in dry mycelia: 2%-6% (W / W).

Description

technical field [0001] The invention belongs to the technical field of bioengineering for preparing beta-carotene by utilizing agricultural and sideline resources, and specifically relates to an improved corn steep liquor for fermenting and producing beta-carotene, a preparation method and application thereof. Background technique [0002] β-carotene (β-carotene) is a kind of carotenoid, which is recognized as a non-toxic, safe and nutritious food fortification by the Food and Agriculture Organization of the United Nations (AFO) and the World Health Organization (WHO). agent. In addition, as an antioxidant, β-carotene has good antioxidant properties, and has obvious functions in the prevention and treatment of many degenerative diseases caused by aging, such as cardiovascular diseases, cataracts and anti-cancer. Furthermore, β-carotene is also used as a food additive. Because it is a fat-soluble compound and its color difference at different concentrations can cover all col...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P23/00C12R1/645
Inventor 蔡俊向梦雄王常高周安盛
Owner HUBEI UNIV OF TECH