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A method for extracting skin tissue from living pufferfish obscura

A technique for puffer obscura and skin, applied in the extraction of puffer obscura skin tissue and post-operative treatment, in the field of puffer obscura live body extraction of skin tissue, which can solve problems such as infection and death, achieve small impact, speed up regeneration, and speed up recovery. The effect of wound healing

Inactive Publication Date: 2016-08-31
FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is generally believed that after cutting the skin, it may lead to infection and death

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] First, use tweezers to lift the skin on one side of the pufferfish obscura, cut a small opening with surgical scissors, and cut about a quarter of the surface area of ​​the side skin from the small opening. When cutting skin tissues of different body colors, try to keep as much connective tissue under the skin as possible to facilitate wound healing. When cutting skin tissue, avoid the blood vessels in the muscle layer, especially the blood vessels at the base of fin rays, to prevent massive bleeding. After the tissue was cut, the wound was smeared with chlortetracycline solution at a concentration of 8:2000 (w / v) to prevent infection. After the color of the chlortetracycline solution becomes light, the puffer pufferfish obscura after surgery can be put into the salt water, and the concentration of the brine is 6‰ (w / v). On the 3rd day after being temporarily raised in salt water, soak the fish again with the same concentration (8:2000, w / v) of aureomycin solution, and...

Embodiment 2

[0016] First, use tweezers to lift the side skin of the pufferfish obscura outwards, cut a small opening with surgical scissors, and cut about one-fifth of the area of ​​the side skin from the small opening. When cutting skin tissues of different body colors, try to keep as much connective tissue under the skin as possible to facilitate wound healing. When cutting skin tissue, avoid the blood vessels in the muscle layer, especially the blood vessels at the base of fin rays, to prevent massive bleeding. After the tissue was cut, the wound was smeared with chlortetracycline solution at a concentration of 9:2000 (w / v) to prevent infection. After the color of the chlortetracycline solution becomes light, the puffer pufferfish obscura after surgery can be put into the salt water, and the concentration of the brine is 7‰ (w / v). On the 3rd day after being temporarily raised in salt water, soak the fish again with the same concentration (9:2000, w / v) of aureomycin solution, and then ...

Embodiment 3

[0018] First, use tweezers to lift the skin on one side of the pufferfish obscura, cut a small opening with surgical scissors, and cut about a quarter of the surface area of ​​the side skin from the small opening. When cutting skin tissues of different body colors, try to keep as much connective tissue under the skin as possible to facilitate wound healing. When cutting skin tissue, avoid the blood vessels in the muscle layer, especially the blood vessels at the base of fin rays, to prevent massive bleeding. After the tissue was cut, the wound was smeared with chlortetracycline solution at a concentration of 10:2000 (w / v) to prevent infection. After the color of the chlortetracycline solution becomes light, the puffer pufferfish obscura after surgery can be put into the salt water, and the concentration of the brine is 8‰ (w / v). On the 3rd day after being temporarily raised in salt water, soak the fish again with the same concentration (10:2000, w / v) of aureomycin solution, a...

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PUM

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Abstract

The invention discloses a method for extracting skin tissue from living pufferfish obscura. First, the epidermis on one side of the living puffer pufferfish obscura is lifted outward, and a small opening is cut with surgical scissors, and the skin tissue is cut from the small opening to the surroundings, and then Use aureomycin solution with a concentration of 8-12:2000 (w / v) to smear the wound. When the color of the aureomycin solution becomes obviously lighter, fish are kept in salt water. After the third day of feeding, the same concentration of aureomycin The fish body was soaked in the aureomycin solution again, and the fish was taken out when the color of the aureomycin solution became obviously lighter, and then reared in the same concentration of salt water. The method of the invention is easy to operate, economical and practical, can non-lethally obtain skin tissues with different body colors from pufferfish obscura, and preserve precious genetic materials while obtaining samples for research.

Description

technical field [0001] The invention relates to a method for extracting skin tissue from living pufferfish, in particular to a method for extracting skin tissue from pufferfish obscura and postoperative treatment, and belongs to the technical field of aquaculture. Background technique [0002] In the prior art, the method of tissue extraction generally needs to kill the fish, and then cut the tissue of a specific part. However, because the germplasm resources of Fugu obscurus are very valuable, especially for the study of some body color mutants, it is necessary to keep as many populations as possible. Prior to this, the traditional method was mostly used for tissue extraction of puffer obscurus. After the fish died, the genetic material disappeared. The research on body color mutants can only be limited to the existing genetic material, which limits the follow-up of follow-up research. The survival rate of Fugu obscurus after cutting its skin is not clear. It is generall...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 金武顾若波闻海波杜兴伟郑金良
Owner FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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