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Method for performing tissue culture inoculation through inoculation box

An inoculation box and tissue culture technology, applied in horticultural methods, botanical equipment and methods, applications, etc., can solve the problems of difficult operation, long inoculation time, low output ratio, etc., and achieve low pollution rate, low cost, The effect of improving efficiency

Inactive Publication Date: 2014-10-15
赵木华
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] In modern tissue culture, most of the ultra-clean workbenches are used as the aseptic inoculation method. The requirements for the number of bacteria in the environmental space are very low, and the operation is difficult. During the inoculation process, the output ratio is low, the inoculation time is long, and pollution high rate

Method used

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Examples

Experimental program
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Effect test

Embodiment

[0017] The method for utilizing the inoculation box to carry out tissue culture inoculation consists of the following steps:

[0018] 1. Use a self-made inoculation box in the shape of a cuboid or prism to replace the ultra-clean workbench for inoculation. The number of bacteria in the overall environmental space is extremely high, and tissue culture can be carried out;

[0019] Second, use the hole method to inoculate:

[0020] (1) Use ordinary glass and aluminum alloy materials on the market to make an inoculation box in the shape of a cuboid or prism. The size of the inoculation box is length × width × height: 66×48×54cm; an ultraviolet sterilizing lamp is placed inside the inoculation box for sterilization Sterilization, so as to achieve a sterile state for inoculation;

[0021] (2) Place special tissue culture bottles with medium in the inoculation box, stack them to form a wall, then place several tissue culture bottles with medium in the box, and place the mother bottl...

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Abstract

The invention discloses a method for performing tissue culture inoculation through an inoculation box. A clean bench is replaced by the self-made cuboid or prismatic-table-shaped inoculation box for inoculation; the inoculation is performed with a cavity method as follows: one horizontally placed tissue culture bottle is firstly inoculated, then the pressure of wall space is formed through the tissue culture bottles, one tissue culture bottle is pulled out, the tissue culture bottle with seedlings inoculated is placed in a cavity, the next tissue culture battle is inoculated, and the process is repeated until the inoculation is finished. The inoculation efficiency is 2-3 times higher than that of the clean bench, in addition, the contamination rate is very low, and the lowest contamination rate is up to 1 / 1000.

Description

technical field [0001] The invention relates to an inoculation method, in particular to a method for tissue culture inoculation using an inoculation box. Background technique [0002] In modern tissue culture, most of the ultra-clean workbenches are used as the aseptic inoculation method. The requirements for the number of bacteria in the environmental space are very low, and the operation is difficult. During the inoculation process, the output ratio is low, the inoculation time is long, and pollution High rate. Contents of the invention [0003] The purpose of the present invention is to provide a method for tissue culture inoculation utilizing an inoculation box. [0004] This method of utilizing an inoculation box for tissue culture inoculation consists of the following steps: [0005] 1. Use a self-made inoculation box in the shape of a cuboid or prism to replace the ultra-clean workbench for inoculation. The number of bacteria in the overall environmental space is ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 赵木华
Owner 赵木华
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