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Method for detecting gallic acid and ampelopsin in tea tincture

A technology of gallic acid and viniferin, which is applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of complex composition, false positives, long analysis time, etc. Good results

Active Publication Date: 2014-10-15
CHINA TOBACCO FUJIAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] At present, the detection method of gallic acid is mainly the detection method of liquid chromatography combined with ultraviolet detector, but the composition of flavor and fragrance samples is complex, and it takes a long analysis time to separate the target substance and the interference substance baseline by liquid chromatography, and there are The problem of false positives
There is no report on the method of simultaneously detecting gallic acid and staphylophyllin

Method used

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  • Method for detecting gallic acid and ampelopsin in tea tincture
  • Method for detecting gallic acid and ampelopsin in tea tincture
  • Method for detecting gallic acid and ampelopsin in tea tincture

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Embodiment 1

[0050] The present embodiment detects the content of gallic acid and staphylophyllin in tea tincture, and the detection method is as follows, and the flow chart of the detection method is as follows: figure 1 shown.

[0051] (1) Weigh 0.1g each of gallic acid and octopus (analytical pure), accurate to 0.0001g, dissolve with methanol and transfer to a 1000mL volumetric flask, and dilute to volume with methanol to obtain standard stock solution of gallic acid and octopus respectively Standard stock solution of steroid; respectively pipette 0.01mL, 0.05mL, 0.1mL, 0.5mL, 1.0mL of gallic acid standard stock solution and steroid standard stock solution to 100mL volumetric flask, and use 10% (v / v) methanol When the aqueous solution is constant volume, the concentrations of gallic acid and staphylophyllin are respectively 0.01μg / mL, 0.05μg / mL, 0.1μg / mL, 0.5μg / mL, and 1.0μg / mL, and the series of standard solutions of gallic acid and staphylophyllin are respectively obtained. Glutin se...

Embodiment 2

[0067] In this embodiment, the repeatability and standard addition recovery of the method of the present invention are tested, and the detection method is as follows.

[0068] The recovery rate of the method of the present invention was determined by the standard addition method. Take by weighing 0.1g of the tea tincture sample to be tested (the sample is the same as the tea tincture sample used in the step (2) of Example 1), each in three parts, add 0.005mL, 0.01mL, 0.02mL, 0.05mL of the example 1 gallic acid standard stock solution and staphylophyllin standard stock solution prepared in step (1), then prepare standard sample solution according to the preparation method in embodiment 1 step (2) " preparation of sample solution ", each sample is respectively Measure 6 times, the condition of chromatographic and mass spectrometry analysis is the same as embodiment 1, calculate the recovery rate of gallic acid and staphyloin in the tea tincture of this method according to the an...

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Abstract

The invention relates to a method for detecting gallic acid and ampelopsin in tea tincture. The method comprises performing determination through a liquid chromatography tandem mass spectrometry and performing calibration through an external standard method. The method for detecting the gallic acid and the ampelopsin in the tea tincture has the advantage s of being short in detecting time, simple in operation, high in sensitivity and recovery rate, good in repeatability and the like. Chromatographic conditions in the method enable the chromatographic peaks of the gallic acid and the ampelopsin to be well separated from those of impurities and to obtain good linearity ranges with limit of detection of 0.002 mu g / mL, the recovery rate of the gallic acid is between 91.3% and 109.2%, the recovery rate of the ampelopsin is between 84.1% and 97.8%, and the relative standard deviations of the detecting results of samples are all smaller than 5%.

Description

technical field [0001] The invention relates to a method for detecting the contents of gallic acid and staphylophyllin in tea tincture, and belongs to the technical field of trace analysis. Background technique [0002] Tea is one of the three major beverages in the world and originated in my country. At present, it is generally believed that the base plants of tea are the tea of ​​the genus Theaceae and its variant big-leaf tea. In taxonomy, both are tea plants. Tea contains a variety of nutrients, such as polyphenols, flavonoids, alkaloids, sugars, and amino acids. [0003] Gallic acid (gallic acid, GA) is a component of hydrolyzable tannins, also known as gallic acid, chemical name 3,4,5-trihydroxybenzoic acid, is a simple phenolic compound with physiological activity in tea. Staphylococcus, also known as dihydromyricetin, is the extract of Vitis vine tea, and is the main active ingredient flavonoids in vine tea. Both gallic acid and staphylophyllin have various biome...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 邓其馨谢卫黄朝章许寒春张廷贵李巧灵张峰
Owner CHINA TOBACCO FUJIAN IND
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