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Molecular marker for regulating main effect QTL of grain length of corn and application of molecular marker

A molecular marker, corn technology, applied in the determination/inspection of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., can solve the problem of no consistent results in grain length QTL analysis, and achieve simple identification methods, high selection efficiency, and selection. targeted effect

Active Publication Date: 2014-12-17
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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AI Technical Summary

Problems solved by technology

Due to the limitations of the type of mapping population (mostly F2:3 families), the precision of the genetic linkage map used (mostly ~200SSR markers), and the evaluation method of grain length (depth), there is no consistent result for the QTL analysis of grain length.

Method used

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  • Molecular marker for regulating main effect QTL of grain length of corn and application of molecular marker

Examples

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Embodiment Construction

[0017] The experimental methods in the following examples are conventional experimental methods unless otherwise specified. The experimental reagents and consumables described in the following examples are from General Biochemical Reagent Company unless otherwise specified.

[0018] In this embodiment, the detailed steps for obtaining the main QTL molecular markers for regulating the grain length of corn are as follows:

[0019] (1) Construction of maize triple test cross (TTC) population and measurement of grain length

[0020] 82 IBM recombinant inbred line populations with high genetic exchange rates of different genotypes were crossed with their parents B73, Mo17 and F1 respectively to obtain TC(B73), TC(Mo17) and TC(F1) triple testcross populations. 82 IBM populations of different genotypes, TC(B73), TC(Mo17) and TC(F1) triple testcross populations were planted in the experimental base according to random grouping. Each genotype material was planted in a single row, wit...

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Abstract

The invention relates to the field of molecular breeding of plants and particularly relates to a molecular marker for regulating main effect QTL of grain length of corn and an application of the molecular marker. The molecular marker for regulating the main effect QTL of grain length of corn consists of two pairs of primers InDel14 and umc1269. A method for assisted selection of long grain corn comprises the following steps: extracting genome DNA of corn to be detected; carrying out PCR amplification on the two pairs of primers InDel14 and umc1269; and if amplification products with the lengths of 236bp and 350bp are obtained, determining the corn to be detected is candidate long grain corn, and applying the candidate long grain corn to breeding. By carrying out assisted selection of long grain corn by means of the molecular marker provided by the invention, the length of the grain can be predicted just by detecting the characteristic amplification strip of the molecular marker, so that the molecular marker is simple in identification method and high in selecting efficiency.

Description

technical field [0001] The invention relates to the field of crop molecular breeding, in particular to a molecular marker for regulating the main effect QTL of corn grain length and its application. Background technique [0002] Corn is an important food crop in the world and has become the second largest crop in my country. With the rapid increase of population and the continuous reduction of arable land, the production of food crops such as corn needs to be greatly increased to meet human needs. The improvement of corn yield is the main technical means to solve the problem of reducing the total amount of grain caused by the reduction of cultivated land. In maize breeding, the improvement of yield per unit area is not only dependent on the increase of planting density, but also the weight of kernels is an important factor affecting yield. [0003] Yield traits are complex quantitative traits controlled by multiple genes. As a direct trait affecting yield, grain traits ca...

Claims

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Application Information

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IPC IPC(8): C12N15/113C12Q1/68
Inventor 张体付赵涵
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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