Molecular marker tightly in linkage with corn line grain number main effect QTL and application thereof

A technology of molecular markers and row kernel number, which is applied in the field of corn breeding and molecular biology, can solve the problems of inconsistent results, etc., and achieve the effect of simple identification method, clear selection target and high selection efficiency

Inactive Publication Date: 2017-06-30
JIANGSU ACAD OF AGRI SCI
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AI Technical Summary

Problems solved by technology

Due to the targeted group type (mostly F 2:3 family), the accuracy of the genetic linkage map used (mostly ~200 SSR markers), and the evaluation method of the number of rows of grains, etc., there is no consistent result for the QTL analysis of the number of rows of grains

Method used

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  • Molecular marker tightly in linkage with corn line grain number main effect QTL and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A molecular marker of the main QTL controlling the number of kernels in a row of maize. The molecular marker is composed of two pairs of primers, InDe176 and umc1854. The sequence of the primer InDel14 is:

[0022] Forward: 5'-TCAGTGCAAAAAGGAATCTCAA-3'

[0023] Reverse: 5'-TGAACGTCTCCCCTTACAATGC-3'

[0024] The sequence of the primer umc1269 is:

[0025] Forward: 5'-TCTAAAGTATGGCTGGAAACGAGC-3'

[0026] Reverse: 5'-GCAACGATAAGTACCAGAATATGGC-3'.

[0027] Extract the genomic DNA of the corn triple testcross population to be tested, and use primers InDe176 and umc1854 for PCR amplification to obtain amplified products with a length of 174bp and 142bp, and the amplified products are passed through 6% polyacrylamide gel and 1% agarose gel. After separation by gel electrophoresis, InDe176 and umc1854 were obtained respectively, and the corn to be tested was the corn with a large number of rows.

[0028] The described maize varieties are B73 and Mo17 and the IBM groups comp...

Embodiment 2

[0032] A detailed step for obtaining the main effect QTL molecular markers for controlling the number of grains in a row of corn is as follows:

[0033] (1) Construction of corn triple test cross (TTC) population and measurement of row kernel number

[0034] 82 high genetic exchange rate IBM recombinant inbred lines were crossed with their parents B73, Mo17 and F1 respectively to obtain TC(B73), TC(Mo17) and TC(F1) triple testcross populations. 82 IBM populations, TC(B73), TC(Mo17) and TC(F1) triple test cross populations were planted in Liuhe Experimental Base of Jiangsu Academy of Agricultural Sciences in 2012 according to random block design, with three replicates. Each genotype material was planted in a single row, with a plant spacing of 30 cm and a row spacing of 60 cm, and normal field management. After emergence, the leaf tissue of the IBM population was collected to extract DNA. After the grains were physiologically mature, the mature ears of IBM recombinant inbred ...

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Abstract

The invention discloses a molecular marker tightly in linkage with main effect QTL of corn line grain number and application thereof. The provided QTL is positioned on a first chromosome 4.09bin, and tightly linked molecular marker consists of primers InDe176 and umc1854. A genome DNA of a triple test-crossed group of corn to be tested is extracted, PCR amplification is carried out by using the primers InDe176 and umc1854, and amplification products having the lengths of 174bp and 142bp are obtained and can be used for predicting the corn line grain number. The molecular marker is used for genotype detection of the corn and its derived varieties or strains to judge the line grain number of the varieties or the strains and improve the selection accuracy and breeding efficiency.

Description

technical field [0001] The invention relates to the fields of corn breeding and molecular biology, in particular to a closely linked molecular marker of main effect QTL controlling the number of grains in a row of corn and an application thereof. Background technique [0002] Corn is an important food crop in the world and has become the second largest crop in my country. With the rapid increase of population and the continuous reduction of arable land, the production of food crops such as corn needs to be greatly increased to meet human needs. The improvement of corn yield is the main technical means to solve the problem of reducing the total amount of grain caused by the reduction of cultivated land. Under a specific planting density, the yield per unit area of ​​corn is determined by the grain yield per ear and the number of ears; and the grain yield per ear is determined by the number of kernels per row (KernelperRow: KPR) and 100-kernel weight. It can be seen that KPR...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 周玲梁帅强赵涵
Owner JIANGSU ACAD OF AGRI SCI
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