Molecular marker tightly in linkage with corn line grain number main effect QTL and application thereof

Abstract

The invention discloses a molecular marker tightly in linkage with main effect QTL of corn line grain number and application thereof. The provided QTL is positioned on a first chromosome 4.09bin, and tightly linked molecular marker consists of primers InDe176 and umc1854. A genome DNA of a triple test-crossed group of corn to be tested is extracted, PCR amplification is carried out by using the primers InDe176 and umc1854, and amplification products having the lengths of 174bp and 142bp are obtained and can be used for predicting the corn line grain number. The molecular marker is used for genotype detection of the corn and its derived varieties or strains to judge the line grain number of the varieties or the strains and improve the selection accuracy and breeding efficiency.

Description

technical field

[0001] The invention relates to the fields of corn breeding and molecular biology, in particular to a closely linked molecular marker of main effect QTL controlling the number of grains in a row of corn and an application thereof. Background technique

[0002] Corn is an important food crop in the world and has become the second largest crop in my country. With the rapid increase of population and the continuous reduction of arable land, the production of food crops such as corn needs to be greatly increased to meet human needs. The improvement of corn yield is the main technical means to solve the problem of reducing the total amount of grain caused by the reduction of cultivated land. Under a specific planting density, the yield per unit area of ​​corn is determined by the grain yield per ear and the number of ears; and the grain yield per ear is determined by the number of kernels per row (KernelperRow: KPR) and 100-kernel weight. It can be seen that KPR...

Images