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A kind of microbial collection method of soil or sediment sample

A collection method and sediment technology, applied in the field of microorganisms, can solve the problems of difficult to ensure low temperature, difficult to transport, difficult to operate with low biomass samples, etc.

Active Publication Date: 2016-05-11
ADVANCED ENERGY & ENVIRONMENTAL TECH INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Low-temperature cryopreservation must ensure that the sample must be strictly guaranteed during the process of low temperature from collection to transportation, and it is often difficult to ensure low temperature in field construction
[0004] 2) Difficulty in transportation
It is difficult to transport frozen samples stored in refrigerators, dry ice or liquid nitrogen from the field to the laboratory
[0005] 3) Low biomass samples are difficult to handle

Method used

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  • A kind of microbial collection method of soil or sediment sample
  • A kind of microbial collection method of soil or sediment sample

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Experimental program
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Effect test

Embodiment 1

[0026] The area involved is a high-altitude meadow area on the Qinghai-Tibet Plateau, which belongs to the permafrost zone, with a small amount of vegetation on the surface, low microbial biomass, and a large number of rocks in the soil samples. In order to carry out exploration of natural gas hydrate and study microbial diversity in this area, it is necessary to lay out large-scale survey lines or survey nets, and to collect a large number of surface soil samples. Using conventional sampling methods, due to the low microbial load, a large number of samples need to be collected at each site, which is time-consuming and difficult to transport, and it is difficult to achieve rapid freezing of samples in high-altitude field areas.

[0027] The specific implementation steps of the sample collection and preservation method with low microbial mass of the present invention are as follows:

[0028] 1. According to the actual exploration situation, select 2 survey lines that have been ...

Embodiment 2

[0032] Embodiment 2: method of the present invention compares with conventional freezing method

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Abstract

The invention relates to a collection and preservation method of soil or sediment microbes in a low-biomass environment. The method comprises the following steps: adding a soil or sediment sample into a high-osmotic pressure alkaline buffer solution which is sterilized, evenly stirring, then standing and collecting supernatant liquid; adding the soil or sediment sample into the supernatant liquid, evenly stirring, then standing and collecting supernatant liquid; repeatedly performing the step 2; and finally, sealing the obtained supernatant liquid, and then preserving the sealed supernatant liquid at room temperature. According to the method provided by the invention, microbial cells are collected in the field via a vibrating and stirring method, so that the operation process is simple, a large number of microbial cells can be collected within short time, and samples are preserved and transported at room temperature. Thus, the method provided by the invention is suitable for collection and preservation of the soil or sediment microbes in environments with a low microbial biomass such as deserts, gobi, permafrost regions, oceans and the like.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a method for collecting microorganisms from soil or sediment samples with low microbial amounts. Background technique [0002] At present, the collection and storage methods of microbial samples for molecular biological analysis are mainly rapid low-temperature freezing after on-site collection, that is, taking a certain amount of soil or sediment samples in the field and sealing them, and then using refrigerators, liquid nitrogen or dry ice to freeze and store them. The whole process of transportation needs to be kept in a low temperature and frozen state. This method has the following problems: [0003] 1) Strict temperature requirements. Low-temperature cryopreservation must ensure that the sample is collected and transported, and the low-temperature process needs to be strictly guaranteed, and it is often difficult to ensure low temperature in field construction. [0004] 2) ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/24
Inventor 张勇邓诗财郝纯梅海
Owner ADVANCED ENERGY & ENVIRONMENTAL TECH INC