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Preparation method and application of polyhydric sterol monomer compound

A technology of polyhydroxysterols and compounds, which is applied in the application field of polyhydroxysterol monomer compounds, can solve the problems such as the anti-artemia activity of chemical components that have not yet been seen, and achieve the effect of simple preparation method, wide source and high efficiency

Active Publication Date: 2016-03-30
广西海洋研究所有限责任公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, there is no report on the anti-artemia activity of its chemical constituents

Method used

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  • Preparation method and application of polyhydric sterol monomer compound
  • Preparation method and application of polyhydric sterol monomer compound
  • Preparation method and application of polyhydric sterol monomer compound

Examples

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Embodiment 1

[0013] Preparation:

[0014] (1) Take fresh wrinkled sea squirt wet weight 1.5kg, homogenize it in a tissue masher, and extract 4 times with 95% ethanol ultrasonic wave for 30 minutes each time. Combine the extracts and concentrate the extracts on a rotary evaporator under reduced pressure to 35 When the relative density of heat measured at ℃ is 1.1-1.4, 1.5g of extract is obtained. Disperse the extract with 1500g of water. After dispersion, it is extracted with the same amount of ethyl acetate. When the extract is concentrated under reduced pressure to 35℃, the relative density of heat measured is 1.1― At 1.4, 700.0 mg of ethyl acetate phase extract was obtained;

[0015] (2) Completely dissolve the ethyl acetate phase extract with methanol, use Sephardax LH-20 gel column chromatography, methanol elution, and then use RP-18 reversed-phase silica gel column chromatography, the methanol / water concentration is 30%, 40%, 50%, 60%, 70%, 80%, 90%, 100% were used for gradient elution. F...

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Abstract

The invention discloses a preparation method of a polyhydroxy sterol monomeric compound and an application. The preparation method comprises the following steps: triturating and uniformly homogenizing fresh styela plicata, carrying out ultrasonic extraction by 95% ethanol, decompressing and concentrating to obtain an extract, dispersing the extract by using water, extracting by virtue of ethyl acetate, and carrying out concentration under reduced pressure on the extraction liquid to obtain an ethyl acetate liquid extract; fully dissolving the ethyl acetate liquid extract by methanol, carrying out SephadaxLH-20 gel column chromatography and RP-18 reverse silica-gel column chromatography; and finally, preparing by using efficient high performance liquid chromatography to respectively obtain a compound 1, dendronesterol A and a compound 2 (20S, 22E, 24R)-ergosta-7, 22-dien-3beta, 5alpha, 6beta-triol, wherein the artemia resistant activity of the compounds 1 and 2 is detected, and the fatality rates to artemia are respectively 87.4% and 82.9% when the concentration is 50 microgramme / mL and rise along with the concentration, showing concentration dependence. The compound can be applied to preparing artemia-resisting drugs. The compound which can be applied to preparing artemia-resisting drugs has easily available raw materials and is wide in prospect.

Description

Technical field [0001] The present invention relates to a method for preparing polyhydroxy sterol monomer compounds, and also relates to the application of polyhydroxy sterol monomer compounds. The polyhydroxy sterol monomer compounds refer to monomer components extracted and separated from sea squirts. It belongs to the field of marine natural product chemistry. Background technique [0002] Since the 1980s, some natural product researchers in the world have discovered compounds with anti-tumor and anti-viral activities from ascidians and many have entered clinical research, making ascidians other than sponges and corals, humans have significant pharmacology. Important marine biological resources of active substances. There are many researches on the nutritional components of ascidians at home and abroad, such as amino acids, mineral elements, fatty acids and polysaccharides, which have considerable benefits to the human body and are called "Northeast Treasures". Styelaplicata...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J9/00A01N45/00A01P7/00
CPCA01N45/00C07J9/00A01N2300/00
Inventor 孙雪萍徐艳黄国强杨家林刘海娟
Owner 广西海洋研究所有限责任公司
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