Pharmaceutical composition for reducing blood fat
A technology for lowering blood lipids and compositions, applied in the field of pharmaceutical compositions for lowering blood lipids, can solve problems such as lack of therapeutic drugs, and achieve a clear effect of lowering blood lipids
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[0012] Example Preparation of magnolia fruit extract
[0013] Take by weighing 1 kilogram of magnolia fruit, add the water that weight is 5 times of described magnolia fruit weight in magnolia fruit, heat 3 hours under the condition of reflux, cool to room temperature, filter, add absolute ethanol to alcohol in filtrate The content is 60% by volume, let it stand for 24 hours, filter, recover the ethanol in the filtrate, then centrifuge at 5500r / min for 5min, remove the supernatant, and vacuum-dry the precipitate obtained through centrifugation at 65°C to constant weight. Instantly.
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[0015] Establishment of hyperlipidemia animal model
[0016] Selection of Pharmacological Indicators
[0017] Select with high-fat feed (20% by weight of lard, 10% by weight of cholesterol, 2% by weight of pig bile salt, 0.2% by weight of propylthiouracil, 20% by weight of propylene glycol, 20% by weight of Tween-80, others are water) The hyperlipidemia model of SD rats was caused by feeding animals, and the drug effect of magnolia fruit extract was investigated with the blood lipid level as the pharmacological index.
[0018] Model building method
[0019] Under the conditions of room temperature 27 ℃ and humidity 60%, after raising the animals for one week, they were divided into blank control group, hyperlipidemia model group, and vehicle control group (DMSO aqueous solution, DMSO and water were mixed and shaken at a volume ratio of 1:4 to Di'ao Xinxuekang (purchased from Chengdu Di'ao Pharmaceutical Group Co., Ltd., approval number: Z10910051, product number: C1400000197...
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