Device and method for extraction and sampling of pesticide residues in fruits and vegetables for microfluidic detection

Abstract

The invention discloses a fruit-vegetable pesticide residue extraction and sampling device and method for microfluidic detection. According to the device and the method, the grinding time and the adding amount of a leaching reagent are automatically calculated according to the weight of fruits and vegetables put into the device; and the automatic adding of the leaching reagent, the automatic discharging of a waste liquid and the microfluidic sampling with a detection reagent are realized by virtue of an internal drawing and pressing structure. An automatic grinding structure is arranged in the device so as to ensure that pesticide residues in the tissues of fruits and vegetables are fully extracted; an ultrasonic energy converter array is arranged in the bottom of the device so as to ensure that the leaching reagent and the tissues of fruits and vegetables are fully mixed in an extraction process and a filter screen cannot be blocked during the leaching and the microfluidic sampling; heating resistance wires are contained in a baseplate so as to ensure that the leaching process meets the temperature standard; and a leaching reagent adding amount formula, grinding time and a temperature control value are set on a control box according to the national standard in China, so that the automatic sampling of the microfluidic detection is realized in strict accordance with the pesticide residue detection standard, and thus the degree of automation of a pesticide residue microfluidic detection technology is greatly improved.

Description

technical field [0001] The invention relates to a microfluidic detection technology, in particular to an automatic extraction and sampling device for pesticide residues in fruits and vegetables for microfluidic detection. Background technique [0002] Microfluidic detection technology can transfer the complex pesticide residue detection process to an automatically completed chip, and because the scale of microfluidic chip is usually nL level, it greatly reduces the consumption of enzymes and other biochemical solutions in the process of pesticide residue detection , in line with the detection requirements of low cost and low consumption, and the automated detection process meets the development needs of popularization and promotion of pesticide residue detection. Therefore, how to effectively apply microfluidic technology to pesticide detection has become the focus of research in this field in recent years. [0003] Chinese patent application number 201410092671.5 discloses...

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Problems solved by technology

[0005] The purpose of the present invention is to address the shortcomings of the lack of automatic pretreatment function in the current microfluidic detection technology for pesticide residues, and propose an automatic extraction and sampling device and method for fruit and vegetable pesticide residues for microfluidic detection, so as to realize fruit, vegetable Automated Extraction and Injection of Pesticide Residues in Vegetable Samples

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