Method for measuring light stability of monascus red pigment

A technology of monascus red pigment and light stability, which is applied in the field of food additives and its detection, can solve the problems of long measurement time, meaningless, inconvenient operation, etc., and achieve the effect of high repeatability, high accuracy and easy operation

Inactive Publication Date: 2015-02-04
西安莹朴生物科技股份有限公司
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Problems solved by technology

Too close can not distinguish the difference of photostability between different monascus pigments, too far and the measurement time is too long, so it is not easy to operate. According to literature reports, a distance of 15-25cm is more suitable
Glass can absorb more than 90% of ultraviolet light, so if the transparent container used in the above literature is made of glass, the method of measuring with ultraviolet light is meaningless

Method used

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  • Method for measuring light stability of monascus red pigment

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Embodiment 1

[0013] First extract the commercially available powdery or pasty monascus pigment with chloroform:methanol=50:50, then pass through the silica gel column soaked in chloroform, and wash with chloroform:methanol=90:10 to remove the polarity Small free pigments were washed with chloroform:methanol=50:50, and the pigments obtained after separation were subjected to thin-layer chromatography on a silica gel plate. The chromatographic agent was chloroform:methanol:water=65:25:4. Then get the separated red, orange and yellow pigments, dissolve the obtained pigments in distilled water respectively to make a certain 0.01% pigment solution, and use distilled water as a blank, and use a UV-visible spectrophotometer at 490nm, 374nm, Measure the initial absorbance of red, orange and yellow pigment solutions at 367nm. Then take a certain amount of pigment solution and place it in a Φ12cm plate. The thickness of the pigment solution in the plate is 4.5mm. Put the plate under a 4OW UV lamp an...

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Abstract

The invention relates to a method for measuring the light stability of a monascus red pigment, and belongs to the field of food additives and detection methods thereof. The method for measuring the light stability of the monascus red pigment comprises the following steps: separating commercially available powdered monascorubin or paste monascorubin to obtain a red pigment, a nacarat pigment and a yellow pigment, respectively dissolving the obtained pigments into distilled water to prepare a pigment solution with certain concentration, and measuring the initial absorbance of the distilled water serving as a blank by an ultraviolet visible spectrophotometer; putting a certain amount of the pigment solution into a plate with the diameter phi of 12cm, putting the plate under a 40W ultraviolet lamp for irradiation at the distance of 15cm, and taking out the plate to measure the absorbance every 20min. The method disclosed by the invention has the characteristics of high accuracy, high repetitiveness, simplicity and convenience in operation and the like.

Description

technical field [0001] The invention relates to a method for measuring the light stability of monascus red pigment, and belongs to the field of food additives and detection methods thereof. Background technique [0002] The photostability of monascus pigment is not high, which is well known. There are roughly the following methods for the determination of its photostability: one is to use the method of sunlight, and prepare a certain concentration of pigment solution in a transparent glass container. Then put it under sunlight, measure the color value of the pigment solution at certain intervals, and divide the color value after irradiation by the color value before irradiation to obtain the preservation rate of the pigment. A more accurate measurement is to convert the solar energy into langeys (500 langleys is equivalent to the energy of one day's sunlight in summer) to measure the intensity of irradiation. The second is to irradiate with ultraviolet light. Place the cont...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/33
Inventor 李勇刘文斌
Owner 西安莹朴生物科技股份有限公司
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