A kind of method and application of cross pollination of celery
A technology for hybrid pollination and celery, applied in the directions of application, botanical equipment and method, plant genetic improvement, etc., can solve the problems of lack of cost, moderate pollination and isolation methods, celery seed strains are difficult to achieve pollen isolation, etc., and achieve low cost. , the effect of preventing the mixing of varieties
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Embodiment 1
[0036] The celery first-generation hybrid variety "Jinrui 1" with CMS traits was used to cross with the target transgenic material "Ventura", and F1 was backcrossed with "Ventura" for 4 consecutive generations to obtain the target CMS male sterile line female. This "C01-3A", "Ventura" became the corresponding maintainer line "C01-3B" of the CMS female parent "C01-3A" after selfing.
[0037] In spring, select the strong celery CMS female parent "C01-3A" and male parent "Qianfang" that have passed vernalization, remove the peripheral old leaves and the upper half of the petiole respectively, and prepare them with 1 million units of veterinary streptomycin Soak the roots with 1000 times the solution, and then plant the CMS female parent and male parent plants in pairs at a distance of 0.5 meters, and keep a distance of 0.8 meters between each pair of plants and adjacent plants. Immediately insert a field recording sign next to each pair of seed plants, step on the topsoil of the ...
Embodiment 2
[0042] The first-generation celery hybrid variety "Kuai Nen 60" with CMS traits was used to cross with the target transgenic material "Youventus", and F1 was backcrossed with "Youventus" for 4 consecutive generations to obtain the target CMS male sterile line The female parent "C10-80A" and "Youventus" became the corresponding maintainer line "C10-80B" of the CMS female parent "C10-80A" after selfing.
[0043] In spring, select the strong celery CMS female parent "C10-80A" and male parent "California Emperor" strains that have passed vernalization, remove the peripheral old leaves and the upper half of the petiole, and prepare them with 1 million units of veterinary streptomycin 1000 times the solution was soaked in the roots, and then the CMS female parent and male parent plants were planted in pairs at a distance of 0.5 meters, and a distance of 0.6 meters was maintained between each pair of plants and adjacent plants. Immediately insert a field recording sign next to each p...
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