Application of kaempferol in drugs for inhibiting recipients' rejection of organ transplantation
A technology of rejection and kaempferol, applied in the field of medicine, can solve the problems of high cost, achieve a wide range of applications, reduce the dosage and duration of use, and benefit long-term high-quality life
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Embodiment 1
[0030] Example 1 Kaempferol significantly prolongs the survival time of islet grafts
[0031] The above 6 groups of mice with different treatments were tested for the survival time of islet transplantation, and the test results were as follows: figure 1 and shown in Table 1.
[0032] Table 1 The mean time of kaempferol itself or combined with cyclosporine to promote the survival of islet grafts in mouse recipients
[0033]
[0034] from figure 1 and as can be seen in Table 1:
[0035] The average survival time of islets of mice in the kaempferol group (27 days) was significantly longer than that of the control group (14 days), n=7-8, P=0.011; indicating that kaempferol can significantly prolong the survival time of islet grafts, Reduced rejection of organ transplantation in recipient mice;
[0036] The average survival time of islets of mice in the low-dose cyclosporine group (CsA 6mg) (35 days) was significantly longer than that of the control group (14 days), n=7-8, P...
Embodiment 2
[0040] Example 2 Kaempferol increases regulatory T lymphocytes (CD4 + FoxP3 + Tregs) number
[0041] After feeding the transplanted and differently treated mice in 6 groups for 4 weeks, the number of regulatory T lymphocytes in the mouse kidneys (including islet transplants) and lymph nodes was quantitatively analyzed by flow cytometry. The analysis results were as follows: figure 2 shown, from figure 2 It can be seen in:
[0042] CD4 in kidney and lymph nodes of Kaempferol mice + FoxP3 + The number of Tregs were (6.4±0.6)×10 4 pcs, (3.4±0.4)×10 4 , which were higher than (4.7±0.4)×10 in the control group 4 (P=0.024), (2.2±0.2)×10 4(P=0.022); indicating that kaempferol can significantly induce and up-regulate the number of regulatory T lymphocytes in mouse kidney and lymph nodes, and reduce the rejection of mouse recipients to organ transplants;
[0043] CD4 in kidney and lymph nodes of mice in low dose cyclosporine group (CsA 6mg) + FoxP3 + The number of Tregs ...
Embodiment 3
[0047] Example 3 Kaempferol inhibits IL-6 gene expression in islet transplantation
[0048] Six groups of mice with different treatments after transplantation were fed for 4 weeks, and the expression of IL-6 gene in the islet grafts of recipient mice was detected. The detection results are as follows: image 3 shown, from image 3 It can be seen in:
[0049] The relative expression level of IL-6 mRNA in the kaempferol group was 1.7 ±0.2, while the relative expression level of IL-6 in the control group was 5.0 ±0.6, and kaempferol significantly down-regulated the expression level of IL-6 mRNA (P=0.006);
[0050] The relative expression of IL-6 mRNA in the kaempferol + low-dose cyclosporine group (Kaempferol + CsA 6mg) was 1.0 ± 0.2, and the relative expression of IL-6 in the low-dose cyclosporine group (CsA 6mg) was 4.5 ± 0.4 , the kaempferol + low-dose cyclosporine group (Kaempferol + CsA 6mg) significantly down-regulated the expression level of IL-6 mRNA compared with the l...
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