A kind of culture dish of tissue block method

A petri dish and chassis technology, which is applied in biochemical equipment and methods, bioreactor/fermenter combination, biochemical instruments, etc., can solve the problems such as difficulty in sticking tissue blocks to the wall, reduce the risk of pollution and improve the success rate effect

A petri dish and chassis technology, which is applied in biochemical equipment and methods, bioreactor/fermenter combination, biochemical instruments, etc., can solve the problems such as difficulty in sticking tissue blocks to the wall, reduce the risk of pollution and improve the success rate effect

CN104450505BInactive Publication Date: 2016-01-13ZHONGSHAN HOSPITAL FUDAN UNIV
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  • A kind of culture dish of tissue block method
  • A kind of culture dish of tissue block method
  • A kind of culture dish of tissue block method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Please refer to figure 1 and figure 2 , figure 1 It is a structural schematic diagram of the culture dish chassis 1 of the present invention, figure 2 It is a structural schematic diagram of an "8"-shaped bracket of the present invention. The culture dish chassis 1 is composed of chassis bottom 2 , chassis side 3 , and "8"-shaped support 4. The bottom surface of the chassis 2 is a circular plane, and a side surface 3 of the chassis is connected above the outer edge of the bottom surface 2 of the chassis. , support connecting portion 42, support tubular portion 43, the support tubular portion is a hollow tubular structure, the support tubular portion 43 is fixed on the inner surface of the chassis side 3 and is perpendicular to the chassis bottom surface 2, the bottom surface of the support tubular portion 43 and the chassis There is a gap of 2-3mm height between the bottom surfaces 2, the bracket connecting portion 42 is a cuboid structure and is perpendicular to ...

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Abstract

The invention relates to a tissue block culture dish. The tissue block culture dish comprises a culture dish chassis, a culture dish top cover and a porous cover; the culture dish chassis comprises a chassis bottom surface, a chassis side surface and an 8-shaped support; the 8-shaped support comprises a support raised part, a support connection part and a support tubular part; the support tubular part is in a hollow tube structure, and the lower surface of the support tubular part is provided with the rectangular raised part; and the edge of the porous cover is provided with notches. By adopting the tissue block culture dish provided by the invention, the problem of difficult adherence to wall during tissue block culture can be solved effectively, the risk of contamination of tissue blocks can be reduced, the success rate of the tissue block culture method can be improved, and the tissue block culture can not be affected during the long-distance transfer of the tissue block culture dish.

Description

【Technical field】 [0001] The invention relates to a vessel for primary culture of cells, in particular to a tissue block method culture vessel. 【Background technique】 [0002] Tissue culture is a method of primary culture of cells. The current tissue block culture methods mainly include the overturning drying method and the thin layer culture method. The main steps of the overturning and drying method are as follows: cut the tissue into a tissue block of about 1 cubic millimeter, wash it and transfer it to a culture bottle, turn the culture bottle over, add an appropriate amount of culture medium to the non-cell growth surface, and let it stand for a period of time to make the tissue block microscopic. After drying, turn the culture bottle over to cover the tissue block with the culture medium; the thin-layer culture method also cuts the tissue into a tissue block of about 1 cubic millimeter, cleans it and transfers it to a culture bottle, and adds a very small amount of cu...

Claims

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Application Information

Patent Timeline
13 Jan 2016
Publication
CN104450505B
IPC
C12M1/22
CPC
C12M21/08; C12M23/10; C12M23/38; C12M23/48
Inventors
王春生; 孙勇新