A one-component tmb chromogenic solution

Abstract

The invention discloses a sing-component TMB color development solution. The color development solution per 1000 mL comprises the following components: 7-10 g of citric acid, 16-20 g of sodium hydrogen phosphate, 0.1-0.5 g of perboric acid soda, 20-30 ml of glycerol, 5-40 mg of TMB and 10-40 ml of DMSO. When the sing-component TMB color development solution provided by the invention is applied to a developing step in an enzyme-linked immunosorbent assay reaction, the process that a solution A and a solution B of the TMB color development solution need to be mixed before use is eliminated, the operation time is shortened, the change of the concentration of effective color development components of the TMB color development solution is avoided, so that the sensitivity of the TMB color development solution is increased; as the solvent of the TMB is DMSO, compared with a conventional alcohol solvent, the TMB dissolving speed is high, and the dissolving is sufficient; the stability of the dissolved solution is better; the color development effect is improved.

Description

technical field [0001] The invention relates to the field of TMB chromogenic solution, in particular to a one-component TMB chromogenic solution. Background technique [0002] TMB is a peroxidase substrate used in ELISA reactions. The substrate produces a soluble light blue end product that can be read on a spectrophotometer at 370 or 635 nm. It is suitable for quantitative and qualitative analysis in the chromogenic stage of ELISA. The principle of the test is: when the enzyme labeled on the antibody or antigen reacts with TMB, hydrogen peroxide, etc. in the chromogenic solution, the solution turns from colorless to yellow after adding the stop solution, and the depth of yellow reflects the amount of antigen-antibody binding in the ELISA system , so as to judge the amount of a certain antigen or antibody. Measure the absorbance value with a microplate reader to judge the degree of immune reaction. [0003] Most of the TMB chromogenic reagent solutions used in the existi...

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