A kind of preparation method of one-component tmb chromogenic solution

A color developing solution, one-component technology, applied in material analysis by observing the influence of chemical indicators, and analysis by chemical reaction of materials, etc., can solve the problems of troublesome operation, high color background, poor stability, etc. problems, to achieve the effect of improving efficiency, simplifying the operation process, and improving stability

Inactive Publication Date: 2017-04-05
CHENGDU VERO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a preparation method of a one-component TMB chromogenic solution to overcome the problems of poor stability, high color background and troublesome operation of the existing TMB chromogenic solution

Method used

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  • A kind of preparation method of one-component tmb chromogenic solution

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Effect test

Embodiment 1

[0023] A kind of preparation method of one-component TMB chromogenic solution, comprises the following steps:

[0024] First, weigh 7g of citric acid, 16g of disodium hydrogen phosphate, and 0.1g of sodium perborate into a 1000mL beaker A, and add 800mL of purified water to dissolve;

[0025] Next, adjust the pH of the solution in beaker A to 4.5, add 20 mL of glycerin, and then add 1 EDTA;

[0026] Then, weigh 5mg of TMB and place it in a 100mL beaker B, add 10ml of ethanol to dissolve it;

[0027] Finally, transfer the solutions in beaker A and beaker B to a 1000mL volumetric flask, add purified water to make up to 1000mL, and shake well.

Embodiment 2

[0029] A kind of preparation method of one-component TMB chromogenic solution, comprises the following steps:

[0030] First, weigh 10g of citric acid, 20g of disodium hydrogen phosphate, and 0.5g of sodium perborate into a 1000mL beaker A, and add 800mL of purified water to dissolve;

[0031] Next, adjust the pH of the solution in beaker A to 6.0, add 30 mL of glycerin, and then add 3 g of EDTA;

[0032] Then, weigh 40 mg of TMB and place it in a 100 mL beaker B, add 30 ml of ethanol to dissolve it;

[0033] Finally, transfer the solutions in beaker A and beaker B to a 1000mL volumetric flask, add purified water to make up to 1000mL, and shake well.

Embodiment 3

[0035] A kind of preparation method of one-component TMB chromogenic solution, comprises the following steps:

[0036] First, weigh 9g of citric acid, 18g of disodium hydrogen phosphate, and 0.2g of sodium perborate into a 1000mL beaker A, and add 800mL of purified water to dissolve;

[0037] Next, adjust the pH of the solution in beaker A to 5.0, add 25 mL of glycerin, and then add 2 g of EDTA;

[0038] Then, weigh 30 mg of TMB and place it in a 100 mL beaker B, add 20 ml of ethanol to dissolve it;

[0039] Finally, transfer the solutions in beaker A and beaker B to a 1000mL volumetric flask, add purified water to make up to 1000mL, and shake well.

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Abstract

The invention discloses a preparation method for a sing-component TMB color development solution. The preparation method comprises the followings steps: respectively weighting 7-10 g of citric acid, 16-20 g of sodium hydrogen phosphate and 0.1-0.5 g of perboric acid soda to be placed in a 1000 mL beaker A, and adding into 800 mL of purified water to enable the mixture to be dissolved; secondly, adjusting the pH of the solution in the beaker A to be 4.5-6.0, adding into 20-30 mL of glycerol, and then adding into 1-3 g of EDTA; thirdly, weighing 5-40 mg of TMB to be placed in a 1000 mL beaker B, and adding into 10-30 mL of alcohol to enable TMB to be dissolved; finally, transferring the solution in the beaker A and the solution in the beaker B to a 1000 mL volumetric flask, adding purified water to set the volume to be 1000 ML, and shaking. Compared with a conventional two-component TMB color development solution, the single-component TMB color development solution prepared by the invention has the advantages of being convenient to operate, high in sensitivity, good in stability and low in color development background.

Description

technical field [0001] The invention relates to the field of TMB chromogenic solution, in particular to a preparation method of one-component TMB chromogenic solution. Background technique [0002] TMB is a peroxidase substrate used in ELISA reactions. The substrate produces a soluble light blue end product that can be read on a spectrophotometer at 370 or 635 nm. It is suitable for quantitative and qualitative analysis in the chromogenic stage of ELISA. The principle of the test is: when the enzyme labeled on the antibody or antigen reacts with TMB, hydrogen peroxide, etc. in the chromogenic solution, the solution turns from colorless to yellow after adding the stop solution, and the depth of yellow reflects the amount of antigen-antibody binding in the ELISA system , so as to judge the amount of a certain antigen or antibody. Measure the absorbance value with a microplate reader to judge the degree of immune reaction. [0003] Most of the TMB chromogenic reagent soluti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78
Inventor 杨秦张志新陶家春叶正义
Owner CHENGDU VERO BIOTECH
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