One-component tmb chromogenic solution for enzyme-linked immunoreaction

Abstract

The invention discloses a one-component TMB developing solution for enzyme-linked immunosorbent assay. Every 1000mL developing solution comprises the following components: 7-10 g of citric acid, 16-20 g of disodium hydrogen phosphate, 0.1-0.5 g of sodium perborate, 20-30 ml of glycerinum, 5-40 mg of TMB and 10-40 ml of DMSO; the one-component TMB developing solution which is good in oxidation effect also comprises 1-3 g of EDTA. Through using the one-component TMB developing solution in the range in the developing state of the enzyme-linked immunosorbent assay, the condition of mixing a solution A and a solution B during the use of the TMB developing solution is avoided, so that the operation time is decreased, the concentration change of the effective developing components of the TMB developing solution is avoided and the sensitivity of the TMB developing solution is improved; the TMB solvent used by the one-component TMB developing solution is DMSO, and compared with the solvent ethyl alcohol, the TMB dissolution speed of the DMSO is high, the dissolution is sufficient and the stability of the dissolved solution is better; through the addition of the EDTA, the stability of the TMB solvent can be further improved.

Description

technical field [0001] The invention relates to the field of TMB chromogenic solution, in particular to a single-component TMB chromogenic solution for enzyme-linked immune reaction. Background technique [0002] TMB is a peroxidase substrate used in ELISA reactions. The substrate yields a soluble light blue end product that can be read on a spectrophotometer at 370 or 635 nm. It is suitable for quantitative and qualitative analysis of the chromogenic stage in ELISA. The principle of the test is: when the enzyme labeled on the antibody or antigen reacts with TMB, hydrogen peroxide, etc. in the chromogenic solution, the solution changes from colorless to yellow after adding the stop solution, and the shade of yellow reflects the amount of antigen-antibody binding in the ELISA system. , so as to determine the amount of a certain antigen or antibody. The degree of immune response was judged by measuring the absorbance value by a microplate reader. [0003] Most of the TMB c...

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a single-component TMB chromogenic solution for enzyme-linked immunoreaction, to overcome the problems of troublesome operation and poor stability of the existing chromogenic solution

Images