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Preparation method of anticancer polysaccharide composition

A technology of composition and polysaccharide, which is applied in the field of preparation of anti-cancer traditional Chinese medicine extracts, can solve the problems of slow onset of effect and low anti-cancer activity, achieve low toxicity and side effects, good anti-cancer activity, and maintain the effect of side effects

Inactive Publication Date: 2015-04-08
HEILONGJIANG UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although traditional Chinese medicine has the advantages of low side effects compared with radiotherapy, chemotherapy, etc., it also has disadvantages such as low anticancer activity and slow onset of effect. Therefore, it is necessary to develop more efficient anticancer traditional Chinese medicine

Method used

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  • Preparation method of anticancer polysaccharide composition
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Examples

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Effect test

Embodiment 1

[0017] Example 1 Preparation method of anticancer polysaccharide composition

[0018] (1) Enzymatic hydrolysis and extraction of polysaccharides: Take 2 parts of Flammulina velutipes and 10 parts of Comfrey, dry them and crush them to 40 mesh, add distilled water and cellulase twice the total weight of Flammulina velutipes and Comfrey, and perform ultrasonic enzymolysis at 37°C for 3 Hours later, the enzymatic solution was obtained;

[0019] (2) Alcohol precipitation and separation of polysaccharides: add absolute ethanol to the enzymatic hydrolysis solution to make the alcohol content reach 80%, let it stand at room temperature for 12 hours, and filter to obtain a precipitate;

[0020] (3) Water-soluble alcohol precipitation purification of polysaccharides; add 10 times the volume of distilled water at 70°C to the precipitate, stir for 30 minutes, filter, discard the precipitate, add absolute ethanol to the supernatant to make the alcohol content reach 80%, and store at room ...

Embodiment 2

[0022] Example 2 Preparation method of anticancer polysaccharide composition

[0023] (1) Enzymatic hydrolysis and extraction of polysaccharides: Take 4 parts of Flammulina velutipes and 5 parts of Comfrey, dry and crush to 40 mesh, add distilled water and cellulase twice the total weight of Flammulina velutipes and Comfrey, and ultrasonically enzymatically hydrolyze 3 parts at 37°C Hours later, the enzymatic solution was obtained;

[0024] (2) Alcohol precipitation and separation of polysaccharides: add absolute ethanol to the enzymatic hydrolysis solution to make the alcohol content reach 80%, let it stand at room temperature for 12 hours, and filter to obtain a precipitate;

[0025] (3) Water-soluble alcohol precipitation purification of polysaccharides; add 10 times the volume of distilled water at 70°C to the precipitate, stir for 30 minutes, filter, discard the precipitate, add absolute ethanol to the supernatant to make the alcohol content reach 80%, and store at room t...

Embodiment 3

[0027] Example 3 Preparation method of anticancer polysaccharide composition

[0028] (1) Enzymatic hydrolysis and extraction of polysaccharides: Take 10 parts of Flammulina velutipes and 2 parts of Comfrey, dry and crush to 40 mesh, add distilled water and cellulase twice the total weight of Flammulina velutipes and Comfrey, and ultrasonically enzymatically hydrolyze 3 parts at 37°C Hours later, the enzymatic solution was obtained;

[0029] (2) Alcohol precipitation and separation of polysaccharides: add absolute ethanol to the enzymatic hydrolysis solution to make the alcohol content reach 80%, let it stand at room temperature for 12 hours, and filter to obtain a precipitate;

[0030] (3) Water-soluble alcohol precipitation purification of polysaccharides; add 10 times the volume of distilled water at 70°C to the precipitate, stir for 30 minutes, filter, discard the precipitate, add absolute ethanol to the supernatant to make the alcohol content reach 80%, and store at room ...

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Abstract

The invention provides a preparation method of an anticancer polysaccharide composition with an excellent anticancer effect. The polysaccharide composition prepared from a flammulina velutipes and radices lithospermi mixture by ultrasonic enzymolysis, and water extraction and alcohol precipitation disclosed by the invention shows excellent inhibiting rate on S180, HL60, A546 and H22, and excellent anticancer effect; the inhibiting rate is obviously superior to that when the flammulinan and lithosperman are independently used; and the anticancer polysaccharide composition can be applied to prevention and treatment of solid tumors and / or blood tumors.

Description

technical field [0001] The invention relates to a preparation method of an anticancer traditional Chinese medicine extract, in particular to a preparation method of an anticancer polysaccharide composition comprising Flammulina velutipes polysaccharide and comfrey polysaccharide. Background technique [0002] Cancer, also known as malignant tumor, is a disease caused by abnormalities in the mechanisms controlling cell proliferation. Along with the deterioration of the environment, cancer has become one of the serious diseases that endanger human health. Currently, the treatment of cancer is usually surgical treatment combined with radiotherapy, chemotherapy and so on. However, most cancer patients are already in the middle and advanced stages when symptoms appear, and surgical treatment usually cannot completely remove the cancer tissue, and the appearance of wounds will cause or strengthen the metastasis of cancer cells, and due to the low resolution of cancer cells and no...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/30A61K31/715A61P35/00C08B37/00
Inventor 张晓娟马育轩王艳丽左冬冬李兰
Owner HEILONGJIANG UNIV OF CHINESE MEDICINE
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