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A kind of maize endophytic Bacillus subtilis and its biocontrol application

A technology of bacillus and subtilis, applied in application, bacteria, biocide and other directions, can solve the problem of unstable control effect and achieve the effect of increasing root vitality

Inactive Publication Date: 2017-07-07
浙江省东阳玉米研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the research on corn biological control at home and abroad is basically only aimed at a single disease, and the control effect is unstable. Therefore, those skilled in the art urgently need the emergence of new multifunctional microbial preparations that can effectively control corn-related diseases.

Method used

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  • A kind of maize endophytic Bacillus subtilis and its biocontrol application
  • A kind of maize endophytic Bacillus subtilis and its biocontrol application
  • A kind of maize endophytic Bacillus subtilis and its biocontrol application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1: Isolation and identification of Bacillus subtilis BS0521

[0017] The strain Bacillus subtilis BS0521 of the present invention is isolated from sweet corn seeds, and the specific operation mode is as follows:

[0018] (1) Sample treatment: prepare Zhefengtian No. 2 corn seeds, rinse them with clean water, and soak them in sterile water at 30°C for 30 minutes;

[0019] (2) Surface sterilization: rinsed with sterile water, placed in 95% alcohol for 3 minutes, rinsed with sterile water for 4 times immediately after disinfection, and at the same time took 120 μL of the last rinse water and applied it to NA medium (beef extract 1g, peptone 5g, yeast extract 5g, sodium chloride 5g, sucrose 10g, agar 20g, water 1000mL), incubate at 28°C for 72h, and test the surface sterilization effect;

[0020] (3) Separation: After cutting the surface sterile material into small pieces with sterilized scissors, transfer it into a centrifuge tube containing 1 mL of sterile water,...

Embodiment 2

[0028] Example 2: Effects of Bacillus subtilis BS0521 on maize root vigor and biomass

[0029] The BS0521 strain was inoculated on the NA medium by the three-district streak method, and a single colony was picked and inoculated into the NB liquid medium, and incubated at 30° C. with shaking at 170 r / min overnight for use. The BS0521 bacterial suspension was centrifuged at 10 000 r / min for 20 min, the supernatant was carefully aspirated, and sterilized by filtration with a 0.22 μm microporous membrane to obtain BS0521 sterile filtrate, which was used for later use.

[0030] The corn seeds were soaked with BS0521 bacterial suspension and sterile filtrate for 30min respectively, then sown, and soaked with water as a control. Each treatment was replicated 5 times with 30 seeds per replicate. Seedling emergence rate was counted 7 days after emergence. Harvest corn plants with roots, wash the soil around the roots of the plants, and absorb water. The root vigor of maize seedlings...

Embodiment 3

[0034] Example 3: Broad-spectrum determination of bacteriostatic activity of Bacillus subtilis BS0521

[0035] The pathogenic target bacteria for the broad-spectrum determination of the bacteriostatic activity of Bacillus subtilis BS0521 were B. The four diseases are currently the most prevalent leaf spot and soil-borne diseases on maize plants. The specific operation method is as follows: the pathogenic bacteria are drawn in a straight line in the middle of the PDA medium, and the two sides of the straight line are inoculated with BS0521 (such as figure 1 As shown), the treatment of only inoculating pathogenic bacteria was used as a control, and each treatment was repeated 4 times, and the growth changes of the colonies were observed, and the degree of antagonism was judged by the size of the inhibition zone.

[0036] The results showed that BS0521 had significant inhibitory effects on maize large leaf spot, small leaf spot and sheath blight, and the maximum inhibition zone ...

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Abstract

The invention discloses a corn endophytic Bacillus subtilis and its biocontrol application, belonging to the technical field of corn disease prevention and control. The strain described in the present invention is Bacillus subtilis BS0521, which is preserved in the China Center for Type Culture Collection, with the starting date of preservation being November 16, 2014, the preservation number is CCTCC NO: M 2014566, and the preservation address is Wuhan University, Wuhan, China. The bacterial strain of the present invention has a growth-promoting effect on healthy corn seedlings, and can increase root activity at the same time, and can be used as a biological fertilizer at the seedling stage of corn; it can inhibit pathogenic bacteria of various diseases of corn under in vitro conditions, and can be used as a biological seed coat The agent can effectively control sheath blight, and can be used as an inducer for corn leaf spot;

Description

technical field [0001] The invention belongs to the technical field of corn disease prevention and control, in particular to an endophytic Bacillus subtilis and its biocontrol application. Background technique [0002] Corn is one of the main food crops in my country, and its planting area and total output are second only to wheat and rice, ranking third. In recent years, corn is not only used for food, but also used as high-quality feed for the development of animal husbandry and an important raw material for light industry and pharmaceutical industry. Therefore, corn plays an important role in the development of the national economy. [0003] In recent years, with the change of climate, the change of cultivation system and the replacement of varieties, and the large-scale planting of high-starch varieties, soil-borne diseases of maize, especially sheath blight, have a tendency to increase year by year. In normal years, the incidence rate is about 10%-30%, and in severe ye...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F11/08A01N63/00A01P3/00C12R1/125
CPCA01N63/00C12N1/205C12R2001/125
Inventor 苏婷王桂跃韩海亮赵福成谭禾平包斐
Owner 浙江省东阳玉米研究所
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