Rice disease resistance gene osseh1 and its cloning method, function identification method and application

A disease-resistant gene and identification method technology, applied in the field of functional identification, rice disease-resistant gene OsSeh1 and cloning methods, can solve the problems of rice Seh1 gene research that has not been reported

Active Publication Date: 2017-04-19
LISHUI UNIV
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Problems solved by technology

So far, the study of rice Seh1 gene has not been reported

Method used

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  • Rice disease resistance gene osseh1 and its cloning method, function identification method and application
  • Rice disease resistance gene osseh1 and its cloning method, function identification method and application
  • Rice disease resistance gene osseh1 and its cloning method, function identification method and application

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Embodiment Construction

[0046] The present invention will be described in detail below with reference to the accompanying drawings and specific embodiments.

[0047] The invention utilizes the rice genome information to mine the Seh1 homologous gene in rice, clone its full-length cDNA sequence, carry out amino acid sequence analysis, subcellular localization and expression analysis in the salicylic acid disease resistance pathway, and analyze the transgenic positive plants. To identify the resistance to sheath blight, to preliminarily clarify the function of Seh1 gene in rice disease resistance, and to provide a genetic theoretical basis for the breeding of broad-spectrum disease-resistant rice varieties.

[0048] Part 1. Rice disease resistance gene OsSeh1 and its cloning method and functional identification method

[0049] Rice disease resistance gene OsSeh1, the computer-readable version of its ribonucleic acid molecular sequence information is:

[0050] ATGGCGGAGCGGCAGGTGGCGGAGCTGGGGGCCGGCGCGGCG...

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Abstract

The invention discloses a rice disease-resistant gene OsSeh1 as well as a cloning method, functional identification method and application of the rice disease-resistant gene OsSeh1. A Seh1 homologous gene in rice is excavated from rice genome information, the overall-length cDNA sequence of the Seh1 homologous gene is cloned, the Seh1 homologous gene is subjected to amino acid sequence analysis, subcellular localization and expression analysis in a salicylic acid disease-resistant approach, and the resistance of a transgenic sun plant to rice sheath blight is identified, so that the function of the gene Seh1 in rice disease resistance is primarily determined, and the genetic theoretical basis is provided for the breeding of broad-spectrum and disease-resistant rice varieties.

Description

technical field [0001] The invention relates to the technical fields of gene cloning, gene expression and function identification in biotechnology, and in particular relates to rice disease resistance gene OsSeh1, a cloning method and a function identification method. Background technique [0002] Rice (Oryza sativa) is an important food crop worldwide [1] , is also a model plant for cereal biology [2,3] . Rice sheath blight (Rice sheath blight) is one of the three major diseases of rice (Oryza sativa) in the world. It has a wide range of occurrence and serious damage, seriously affecting the yield and quality of rice [1] . So far, no rice varieties that are completely immune or highly resistant to rice sheath blight have been found in rice germplasm resources. Sheath blight is caused by fungi, which can occur from the rice seedling stage to the heading stage, infecting various tissues including leaf sheaths, leaves, and even ears, resulting in a decrease in seed setting...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C12N15/10C12N15/82A01H5/00
Inventor 夏更寿张燕
Owner LISHUI UNIV
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