Three-dimensional cell assembly device and method based on movable electrode

A technology of three-dimensional assembly and moving electrodes, which is applied in the methods of stress-stimulated microbial growth, biochemical cleaning devices, biochemical equipment and methods, etc. The gel shape is difficult to precisely control and other problems, to achieve the effect of simple structure, easy shape control and reasonable design

Inactive Publication Date: 2015-05-06
XIAN UNIV OF SCI & TECH
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Problems solved by technology

However, in the traditional preparation method, Ca 2+ It is difficult to control the concentration and distribution of the mixed solution
Therefore, the shape of the hydrogel containing cells is also difficult to precisely control, resulting in the growth of cells in three-dimensional space is also difficult to control

Method used

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  • Three-dimensional cell assembly device and method based on movable electrode

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Embodiment Construction

[0030] Such as figure 1 The shown three-dimensional cell assembly device based on movable electrodes includes a conductive bottom plate 4, a conductive needle tube located above the conductive bottom plate 4, a liquid injection device that transports the cell fixative solution containing biological cells into the conductive needle tube, and drives the conductive needle tube. A needle tube moving mechanism for the conductive needle tube to move above the conductive bottom plate 4, and the conductive needle tube is installed on the needle tube moving mechanism. The conductive needle tube includes an inner sleeve 3 and a conductive metal layer 3-1 covering the outer wall of the inner sleeve 3. The upper end of the inner sleeve 3 has a liquid inlet and its lower end has a liquid outlet. The liquid injection device is connected with the liquid inlet of the inner casing 3 through a connecting pipe. The cell fixative is an aqueous solution uniformly mixed with sodium alginate, calci...

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Abstract

The invention discloses a three-dimensional cell assembly device based on a movable electrode. The device comprises a conductive bottom plate, a conductive needle tube coating the conductive bottom plate with a mixed solution, a liquid injection device and a needle tube moving mechanism or a bottom plate moving mechanism, wherein the conductive needle tube comprises an inner sleeve tube and a conductive metal deposition layer covering an outer side wall of the inner sleeve tube; the conductive bottom plate and the conductive metal deposition layer are connected with two power supply output ends of a current controller respectively. The device is simple in structure, reasonable in design, easy and convenient to use and operate and good in use effect, and can simply and conveniently adjust the shape of hydrogel containing cells. Meanwhile, the invention further discloses a three-dimensional cell assembly method based on the movable electrode. The method comprises the following steps: 1, conveying the mixed solution; 2, performing water electrolysis and generating the hydrogel; 3, adjusting the position of the conductive needle tube and adjusting water electrolysis current. The method is simple in step, convenient to realize and good in use effect, and the hydrogel shape is adjusted by controlling the position of the movable electrode and the current.

Description

technical field [0001] The invention belongs to the technical field of biomanufacturing, and in particular relates to a three-dimensional cell assembly device and method based on movable electrodes. Background technique [0002] A key problem to be solved in bioengineering is how to assemble biological cells into biologically active artificial substitutes to maintain, restore or improve the functions of human tissues. In order to better understand how cells grow and develop into functional tissues and organs, existing methods generally study the growth characteristics of cells by culturing them on petri dishes. In recent years, more and more studies have shown that the growth state of cells on a two-dimensional plane cannot truly reflect the growth environment of cells in the human body. Therefore, how to fix biological cells in three-dimensional space and let them grow is a key technology in bioengineering, that is, to fix biological cells and form a three-dimensional fixe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/42C12M1/00C12N13/00C12N5/00
Inventor 尚万峰申亚京
Owner XIAN UNIV OF SCI & TECH
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