Assay method of ligusticum wallichii fingerprint

Abstract

The invention relates to an assay method of a ligusticum wallichii fingerprint. The assay method comprises the following steps: (1) grinding ligusticum wallichii medicinal materials into medium powder, accurately weighing, adding ethyl alcohol, carrying out ultrasonic extraction, chilling, complementing lost solvent weight, filtering through a microporous filtering membrane and taking subsequent filtrate to obtain a test article solution; (2) preparing a reference substance solution; and (3) accurately sucking up the test article solution and the reference substance solution respectively, injecting into a high-performance liquid chromatograph, and measuring, so as to obtain the ligusticum wallichii fingerprint. The assay method has a positive role in effective feeding guidance of crude drugs in clinical medication and production processes and insurance of the reliability of the quality, and is convenient and fast to operate, so as to obtain a similarity result; the quality of the traditional Chinese medicinal material ligusticum wallichii is evaluated; and the conclusion is relatively objective and accurate.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine fingerprints, in particular to a method for measuring the fingerprints of Rhizoma Chuanxiong. Background technique [0002] Chuanxiong is a traditional Chinese medicine recorded in the Chinese Pharmacopoeia. Chuanxiong is the dried rhizome of Ligusticum chuanxiong, an umbrella plant. Ligusticum chuanxiong contains a variety of chemical components: (1) alkaloids, ligustrazine (tetramethyl-pyrazine), ryegrass (Perlolyrine), leucylphenylalanine anhydri-de, glandular Adenine, Trimethylamine, Choline, etc.; (2) volatile oil, Ethyl pentadecanoate, Ethyl palmitate, Ethyl heptadecanoate heptadecanoate), ethyl isoheptadecanoate (Ethyl isoheptadecanoate), ethyl octadecanoate (Ethyl octadecanoate), etc.; (3) Lactone compounds, butylphtha-lide, butenyl-lide (Butylidene phthalide), ligustilide (Ligustilide), neocnidilide (Neocnidilide). Senkyunolide (senkyunolide) A, B, C, E, F, I, etc.; (4) orga...

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Problems solved by technology

The Journal of Nanjing University of Traditional Chinese Medicine, No. 2, 2009, reported the research results of Xu Lanlan et al. "HPLC Fingerprint Study of Rhizoma Chuanxiong Medicinal Material". In the paper, only one compound of ferulic acid was identified; from the above research, it can be seen that only one component or Monitoring of a class of ingredients cannot truly reflect its intrinsic quality

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