Anchor for fixing tissue slice in in-vitro electrophysiological experiment

Abstract

The invention belongs to the technical field of apparatuses and instruments for medical treatment experiment, specifically relates to an anchor for fixing a tissue slice in an in-vitro electrophysiological experiment, and aims at solving the technical problems of the existing anchor for fixing the tissue slice in the in-vitro electrophysiological experiment that the brain tissue cannot be closely contacted with an electrode, the brain tissue cannot be prevented from being flushed away by circularly supplied nutrient perfusate, and the experiment record is greatly interfered. According to the technical scheme, the anchor for fixing the tissue slice in the in-vitro electrophysiological experiment comprises an anchor frame formed by integrating two arc sections and two clamping lugs, and a fixing screen which is arranged between the two arc sections of the anchor frame. The anchor has the advantages of enabling close contact of the tissue slice and the electrode, preventing the tissue slice from being flushed away by circularly supplied nutrient perfusate, and avoiding the interference to the experiment record.

Description

technical field [0001] The invention belongs to the technical field of instruments for medical experiments, and in particular relates to an anchor for fixing tissue slices for in vitro electrophysiological experiments. Background technique [0002] The MED64 planar microelectrode array recording system is a multi-channel in vitro extracellular neurophysiological research tool produced by Alpha Med Science Company of Japan. The system includes a complete set of hardware equipment consumables and analysis software required for in vitro electrophysiological research. Users can conveniently and quickly perform electrophysiological detection on isolated materials such as brain slices and cultured nerve cells; they can measure local electrical changes directly adjacent to the outside of cells, and record the electrophysiological signals of multiple cells in parallel. The MED64 device can record and stimulate different sites at the same time; and the MED64 recording is non-invasiv...

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Problems solved by technology

[0003] The purpose of the present invention is to solve the problem that the existing anchors for fixing tissue slices used in in vitro electrophysiological experiments cannot effectively bring the brain tissue into close contact with the electrodes, and cannot avoid the brain tissue being washed away by the perfusate that circulates nutrients and causing damage to the experimental records. The technical problem of huge interference, providing an anchor for fixing tissue slices for in vitro electrophysiological experiments

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