Molecular marker for regulating main effect QTL (quantitative trait loci) of hundred-grain weight of corn and application of molecular marker

A molecular marker, 100-grain weight technology, applied in the field of genetic engineering, can solve the problems of complex genetic background, affecting the accuracy of test results, unfavorable main effect QTL fine mapping and cloning, etc.

Inactive Publication Date: 2015-06-03
HENAN AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0003] Early genetic groups are mostly F 2 , F 2:3 Primary populations such as , BC1, DH, and RIL can only be used for QTL analysis of crop traits, and minor QTLs are generally not

Method used

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  • Molecular marker for regulating main effect QTL (quantitative trait loci) of hundred-grain weight of corn and application of molecular marker
  • Molecular marker for regulating main effect QTL (quantitative trait loci) of hundred-grain weight of corn and application of molecular marker
  • Molecular marker for regulating main effect QTL (quantitative trait loci) of hundred-grain weight of corn and application of molecular marker

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Embodiment Construction

[0029] The present invention will be further described below in conjunction with specific examples. It should be understood that these examples are only for the purpose of illustration, and in no way limit the protection scope of the present invention.

[0030] 1. SSR primer design

[0031] According to the principle of primer design, use the software Primer 6.0 to obtain the primer sequence, and then send the sequence to the company for synthesis.

[0032] 2. Using SDS method to extract leaf DNA

[0033] (1) When the leaves of corn 3 were unfolded, 5 leaves with the same growth were taken from each area, and stored in a -80°C refrigerator.

[0034] (2) Neatly stack 5 fresh leaves in each area, evenly remove about 5 g of fresh leaves, put them into a mortar filled with liquid nitrogen, grind them into powder quickly, and pour them into a 2.0 mL Beckman centrifuge tube;

[0035] (3) Add 600 μL of SDS extract preheated to 65°C;

[0036] (4) Add 20 μL of β-mercaptoethanol (1% ...

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Abstract

The invention discloses a molecular marker for regulating main effect QTL (quantitative trait loci) of hundred-grain weight of corn and application of the molecular marker. By taking general DNA of corn as a template, PCR amplification is carried out on SSR primers Sym035 and Sym119 to obtain DNA fragments which are 165 bp and 147 bp long through gel electrophoresis; a QTL for regulating hundred-grain weight of corn exists between the SSR molecular markers Sym035 and Sym119 on a Bin5.06 region on the fifth chromosome. By using similar F2: 3 colony, QTL analysis on grain length, grain width and grain thickness show that a QTL for grain width is positioned between the molecular markers Sym024 and Sym128, and the genetic distance is 13.1cM. Correlation coefficients between hundred-grain weight and grain length, grain width and grain thickness in a segregation population are respectively 0.532, 0.726 and 0.187. Reduction of hundred-grain weight of single segment substitution lines SSSL-Z22 is mainly induced by reduction of grain width.

Description

technical field [0001] The invention relates to an SSR molecular marker linked with the main effect QTL of the 100-kernel weight of corn and its application in assisted breeding of the main QTL molecular marker of the 100-kernel weight main effect of the corn. It belongs to the technical field of genetic engineering. Background technique [0002] As a food, feed and industrial raw material crop, corn plays an important role in world agriculture. Demand for maize is expected to exceed that for wheat and rice in 2020 (Ping PL & Pandey S2001). However, maize yields per unit area remain low in most developing countries. Studying the genetic control mechanism of maize yield traits is of great significance for further improving maize yield. [0003] Early genetic groups are mostly F 2 , F 2:3 Primary populations such as , BC1, DH, and RIL can only be used for QTL analysis of crop traits, and minor QTLs are generally not detected, and their genetic background is complex, which...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 席章营冯园园王顺友白青河夏雪陈春侠陆明洋
Owner HENAN AGRICULTURAL UNIVERSITY
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