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Anti-hyperuricemia active ingredient group of polygonum cuspidatum and preparation method and application thereof

A technology of hyperuricemia and active ingredients, applied in the field of medicine, can solve the problems of lack of in-depth research and reduction of the basic system of active substances, achieve the effect of inhibiting xanthine oxidase activity, simple process, and low production cost

Inactive Publication Date: 2015-06-24
QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Polygonum cuspidatum extract significantly reduces blood uric acid levels and inhibits xanthine oxidase activity in animal models of hyperuricemia, and can improve the pathological changes of gouty arthritis (Lin Jinchao. Asia Pacific Traditional Medicine, 2012, 8 (11): 21-22; Hou Jianping . Pharmacology and Clinic of Chinese Medicine, 2010, 26 (5): 76-78; Wang Bin, Hou Jianping, Li Min, Meng Jianguo, Sun Jianning. Pharmacology and Clinic of Chinese Medicine, 2008, 19 (6): 434-437), but lack of activity In-depth study of material basis system

Method used

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  • Anti-hyperuricemia active ingredient group of polygonum cuspidatum and preparation method and application thereof
  • Anti-hyperuricemia active ingredient group of polygonum cuspidatum and preparation method and application thereof
  • Anti-hyperuricemia active ingredient group of polygonum cuspidatum and preparation method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Determination of Cassina-8-O-β-D-glucopyranoside, Emodin-6-O-β-D-glucopyranoside and Emodin-8 in Polygonum cuspidatum Antihyperuricemia Active Components by HPLC -O-β-D-glucopyranoside and Emodin-8-O-β-D-glucopyranoside content method

[0033] Chromatographic conditions and system suitability test

[0034] Octadecylsilane-bonded silica gel was used as filler; acetonitrile was used as mobile phase A, 0.1 formic acid solution was used as mobile phase B, linear gradient elution: 0 min (A 15%, B 85%) → 20 min (A 20%, B 80%) → 35 min (A 30%, B 70%) → 55 min (A 50%, B 50%) → 80 min (A 60%, B 40%) → 85 min (A 100% , B 0%) → 95 min (A 100%, B 0%); flow rate: 1.0 mL min -1 ; The detection wavelength is 280 nm. The number of theoretical plates is calculated based on the peak of emodin-8-O-β-D-glucopyranoside, not less than 4000.

[0035] Solution preparation

[0036] Preparation of reference solution

[0037] Precise weighing depends on cassinone-8-O-β-D-glucopyra...

Embodiment 2

[0043] Take 300 Kg of Polygonum cuspidatum rhizome and root powder, add 9 times the amount of ethanol to reflux for extraction 3 times, each time for 2 hours, filter, combine the extracts, and recover the extract under reduced pressure. Take the extract and dissolve it in water, add it to HPD100 macroporous adsorption resin column, elute with water until the eluate is almost colorless, discard the eluent, and elute with 20% ethanol until the eluent is almost colorless, discard the eluent The liquid was removed and eluted with 60% ethanol until the eluate was nearly colorless, the eluate was collected, and recovered under reduced pressure to obtain 457 g of anti-hyperuricemia active ingredients of Polygonum cuspidatum. Adopt the method measurement of embodiment 1, contain the cassia ketone-8-O-β-D-glucopyranoside of 5.15% weight, the emodin-6-O-β-D-glucopyranoside of 20.64% weight, 32.26% by weight of emodin-8-O-β-D-glucopyranoside and 14.20% by weight of emodin-8-O-β-D-glucopy...

Embodiment 3

[0045] Take 300 Kg of Polygonum cuspidatum rhizome and root coarse powder, add 12 times the amount of 90% ethanol to reflux and extract for 3 times, each time for 2 hours, filter, combine the extracts, and recover the extract under reduced pressure. Take the extract and dissolve it in water, add it to HPD200 macroporous adsorption resin column, elute with water until the eluent is almost colorless, discard the eluent, and elute with 20% ethanol until the eluent is almost colorless, discard the eluent The liquid was removed and eluted with 60% ethanol until the eluate was nearly colorless, the eluate was collected, and recovered under reduced pressure to obtain 479 g of anti-hyperuricemia active ingredients of Polygonum cuspidatum. Adopt the method measurement of embodiment 1, contain the cassia ketone-8-O-β-D-glucopyranoside of 5.86% weight, the emodin-6-O-β-D-glucopyranoside of 22.57% weight, 30.20% by weight of emodin-8-O-β-D-glucopyranoside and 11.15% by weight of emodin-8-...

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Abstract

The invention provides an anti-hyperuricemia active ingredient group of polygonum cuspidatum and a preparation method thereof. The anti-hyperuricemia active ingredient group contains 5-20wt% of cassia ketone-8-O-beta-D-glucopyranoside, 20-30wt% of rheum emodin-6-O-beta-D-glucopyranoside, 30-50wt% of rheum emodin-8-O-beta-D-glucopyranoside and 10-20wt% of physcion-8-O-beta-D-glucopyranoside; the invention further discloses an application of the anti-hyperuricemia active ingredient group of polygonum cuspidatum in preparation of products for treating hyperuricemia.

Description

technical field [0001] The invention belongs to the field of medicine, and relates to anti-hyperuricemia active ingredients of Polygonum cuspidatum and a preparation method thereof, and also relates to the use of anti-hyperuricemia active ingredients of Polygonum cuspidatum in preparing products for treating hyperuricemia. Background technique [0002] Hyperuricemia is a group of heterogeneous diseases caused by purine metabolic disorders or (and) decreased uric acid excretion. It is an important biochemical basis for gout. It is a disease that seriously affects public health. Epidemiological surveys from China, the United States, the United Kingdom and other countries have shown that the incidence of hyperuricemia is rising rapidly, and it has been listed by the World Health Organization as one of the top ten chronic human diseases in the 20th century. Hyperuricemia not only seriously affects the patient's quality of life, but even threatens the life of the patient, seriou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/704A61P19/06C07H15/244C07H15/203C07H1/08
Inventor 王威高华刘小红韩立春刘坤
Owner QINGDAO UNIV
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