A pretreatment method for nucleoside components of Armillaria chrysanthemum fruiting bodies

Abstract

The present invention relates to a pretreatment method for the nucleoside components of Armillaria chrysanthemum fruiting bodies, the method comprising the following steps: (1) Extraction: After the Armillaria chrysanthemum fruiting bodies are crushed, analytical pure water is added for extraction, after filtration, decompression Dry to paste to obtain Armillaria chrysanthemum fruiting body aqueous extract; (2) Alcohol precipitation: Add water to the aqueous extract of Armillaria chrysanthemum fruiting body to dissolve, then carry out alcohol precipitation, filter, and dry under reduced pressure to obtain a brown-yellow extract (3) Resin column chromatography enrichment: add ethanol solution to the brownish yellow extract to dissolve, and separate through resin column, carry out gradient elution with aqueous solution and ethanol solution respectively, and each eluate obtains 20% ethanol solution respectively after drying under reduced pressure The eluate, 50% ethanol solution eluate, 75% ethanol solution eluate and 95% ethanol solution eluate can obtain nucleoside target components. The invention has the advantages of simple process, easy large-scale implementation, repeatability, stable and controllable quality.

Description

technical field [0001] The invention relates to the technical fields of medicinal chemistry and biomedicine, in particular to a pretreatment method for nucleoside components of Armillaria chrysanthemum fruiting bodies. Background technique [0002] Armillaria chrysanthemum, commonly known as chanterelle, is a representative of rare wild fungi in alpine grasslands. The fruiting body of Armillaria chrysanthemum is rich in protein, amino acid, vitamin, mineral, volatile oil and polysaccharide and other nutrients, especially the high content of "selenium", which is one of the excellent plateau treasures. Studies have shown that the fruiting bodies of Armillaria chrysogenum have good antioxidant and antitumor activities. At present, the chemical components isolated and identified from the fruiting body of Armillaria chrysogenum mainly include nucleosides and sterols, among which nucleosides are expected to become the index chemical components of this raw material and one of its ...

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Problems solved by technology

[0003] At present, it has been reported that antioxidant active components have been isolated from Armillaria chrysanthemum (Li Shifeng, Chen Guichen, Bi Yurong. Antioxidant and antitumor activities of two wild edible fungi, Chinese Edible Fungus. 2004, 24(3): 58-63), the study reported that alcohol-soluble components and water-soluble polysaccharide components with good antioxidant activity were obtained by multiple solvent distribution methods, in which the number of solvent distributions was 4-12 times, the process was cumbersome and not suitable for large-scale Production

However, there is no literature report on the research on the pretreatment method of the nucleoside components of Armillaria chrysanthemum fruiting body

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