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A kind of roundworm miRNA inhibitor and application thereof

An inhibitor, a technology for Ascaris suum, applied in the field of biological preparations, can solve problems such as drug residues, and achieve the effects of reducing abundance, reducing infection rate, and avoiding the problem of parasite drug resistance

Active Publication Date: 2018-05-01
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The object of the present invention is to provide a roundworm miRNA inhibitor aiming at the problem of parasite drug resistance caused by existing anti-helminth drugs, as well as the problem of drug residues in animal products and the environment

Method used

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  • A kind of roundworm miRNA inhibitor and application thereof
  • A kind of roundworm miRNA inhibitor and application thereof
  • A kind of roundworm miRNA inhibitor and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Ascaris miRNA inhibitor of the present invention inhibits effect experiment on ascaris:

[0022] 1. Preparation of Tertiary Larvae of Ascaris suum

[0023] Collect Ascaris suum adult females from the small intestine of naturally infected slaughtered pigs, dissect and remove about 1 / 2 of the fork end of the uterus, take out the eggs, put them into a mortar, and wash them with sterilized PBS solution (Na 2 HPO 4 12H 2 O 9.670g; KH 2 PO 4 1.429g; NaCl2.125g; H 2 O 500ml) was slightly ground in a mortar, washed with PBS solution and 1.5% formalin solution, centrifuged twice, discarded the supernatant, put the precipitate into a petri dish, and cultivated in a 28°C incubator for 28 days. The third-stage larvae of Ascaris suum were obtained by shelling with 7.5% hypochlorous acid.

[0024] 2. Preparation of culture medium

[0025] Prepare DMEM medium containing 50% fetal bovine serum, and add penicillin (1000U / ml), streptomycin (1mg / ml) and amphotericin (10μg / ml) to i...

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Abstract

The invention provides a roundworm miRNA inhibitor, the nucleotide sequence of the inhibitor is: UCUACCCGAGAAUCGAACAGUG. The inhibitor of the present invention significantly reduces the abundance of asu-miR-100b by combining with asu-miR-100b, and significantly affects the development of third-stage larvae of Ascaris suum and reduces the infection rate. The asu-miR-100b inhibitor expressed in the whole developmental period of roundworms provided by the present invention can be applied to functions affecting the growth and development of third-stage larvae of Ascaris suum and human roundworms or related to their infectivity.

Description

technical field [0001] The invention belongs to the technical field of biological preparations, and in particular relates to an ascaris miRNA inhibitor and an application thereof. Background technique [0002] Ascaris suum ( Ascaris suum ) is one of the common parasitic nematodes in pigs, which belongs to Ascaridae and lives in the small intestine of pigs. Ascariasis suum is prevalent widely, which greatly endangers the development of pig industry, especially in pig farms with poor sanitation, the infection rate is very high. The main manifestations of sick pigs are poor growth and development, and severe cases can cause growth and development stagnation, or even death. The long-term overuse of anti-helminth drugs has caused the problem of drug resistance in parasites distributed globally and the problem of drug residues in animal products and the environment. Therefore, it is imminent to speed up the research on Ascaris suum and find new ways to effectively control Ascar...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K48/00A61K31/7088A61P33/10
Inventor 徐民俊邵长春朱兴全冯胜勇
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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