Bacillus subtilis fermentation production technology

A Bacillus subtilis and production process technology, which is applied in the field of Bacillus subtilis fermentation production process, can solve the problems of low fermentation level, restricted promotion of microbial water body repairing agents, slow growth rate of bacteria, and the like, and achieves simple fermentation process and broad production. Application prospect, low cost effect

Inactive Publication Date: 2015-09-16
ZHEJIANG SCI-TECH UNIV
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AI Technical Summary

Problems solved by technology

At present, the industrial production process of Bacillus subtilis generally has defects such as slow gro

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (1) Inoculate 25 ul of cryopreserved Bacillus subtilis strains into 3 ml of LB medium (pH6.5), and activate on a shaking table (200 rpm) at 37°C for 20 hours; inoculate 3 ml of activated primary seeds Continue to activate 12h in the improved culture medium of 50ml as secondary fermentation seed. The composition and concentration of the improved medium are (1L): glucose 5g, yeast extract 5g, peptone 15g, KH 2 P0 4 1.5g, K 2 HP0 4 .3H 2 O 1g, (NH 4 ) 2 S0 4 2g pH 6.5. Culture conditions: shaker culture (250 rpm), temperature 37°C.

[0022] (2), the secondary seed of 50ml activation is inoculated in the 5L fermenter culture medium that contains 3L culture medium and cultivates, and temperature is 37 ℃, and dissolved oxygen is controlled at 30% correlation speed (300~800rpm), and pH is controlled at 6.5, Samples were taken every 12 hours to determine the wet weight of the bacteria, and the incubation time was 48 hours. The composition of the fermentation medium: b...

Embodiment 2

[0025](1) Inoculate 50 ul of the cryopreserved bacteria into 20 mL of LB medium at pH 7, and culture it on a shaker (200 rpm) at 37° C. for 24 hours to activate. Inoculate 10ml of activated primary seeds into 300ml of improved medium and continue to activate for 15h. Culture conditions: shaker culture (250 rpm), temperature 37°C. The composition and concentration of the improved medium (1L) are: glucose 5g, yeast extract 5g, peptone 15g, KH 2 P0 4 1.5g, K 2 HP0 4 .3H 2 O 1g, (NH 4 ) 2 S0 4 2g pH 6.5.

[0026] (2), inoculate the secondary seed of 300ml activation on the 15L fermenter tank that contains 10L fermentation medium and cultivate, culture condition: 37 ℃, control dissolved oxygen at 20%, every 12 hours take a sample and measure the thalline concentration, wait to dissolve When the oxygen value rises from 0% to 30%, the dissolved oxygen reverse correlation feeding is started, that is, the dissolved oxygen is higher than 30%, and the fed-batch feeding is starte...

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Abstract

The invention relates to a bacillus subtilis fermentation production technology, which comprises the following steps: 1)inoculating bacillus subtilis in an aseptic culture tube, performing shaking culture and activating to obtain liquid as a first fermentation seed liquid; 2)inoculating first fermentation seed liquid in an improvement medium, continuously performing shaking culture and activating to obtain liquid as a secondary fermentation seed liquid; 3)inoculating the activated secondary seeds in a fermentation medium for fermentation culture, culturing in a fermentation cylinder; and 4)centrifuging a broth at 4000-8000rpm and collecting thalline, and drying at temperature of 30-50 DEG C. The fermentation production technology has the beneficial effect that glucose is employed as a main medium component of carbon source, and thalline growth rate can be grearly increased by continuous feeding and fermentation through dissolved oxygen linkage at anaphase. The bacillus subtilis thalline powder has the advantages of low cost, simple fermentation technology and short production period, and has wide production application prospect.

Description

technical field [0001] The invention relates to bacillus subtilis, in particular to a fermentation production process of bacillus subtilis. Background technique [0002] my country is a big country engaged in aquaculture in the world, and aquaculture accounts for an important proportion of agricultural production. With the continuous improvement of the intensification of the breeding industry, the physical, chemical and ecological environment of the breeding water body is also deteriorating; at the same time, the discharge of domestic sewage and industrial wastewater has also exacerbated the decline in the quality of the breeding water body; these problems have caused the breeding diseases to become more serious. The loss is huge. [0003] The microbial environmental restoration method is a green environmental treatment method developed in recent years, which has the characteristics of short restoration time, simple operation, low cost, and environmental friendliness. Baci...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/125
CPCC12N1/20
Inventor 陈玮陈海敏梁宗锁盛清杨建林王海荣胡秀芳刘立丽
Owner ZHEJIANG SCI-TECH UNIV
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