Bone marrow smear atypical lymphocyte staining kit and application method thereof

Abstract

The invention provides a bone marrow smear atypical lymphocyte staining kit and an application method thereof, wherein the kit comprises a reagent tube filled with Polymer Helper, a reagent tube filled with PBS phosphate buffer solution, a reagent tube filled with antigen retrieval buffer, a reagent tube filled with hydrogen dioxide solution, a reagent tube filled with diaminobenzidine staining solution, a reagent tube filled with hematoxylin staining solution, a reagent tube filled with 1% acidulated alcohol, a reagent tube filled with 1% ammonium hydroxide, a reagent tube filled with xylene, a reagent tube filled with ethyl alcohol, a reagent tube filled with CD3 monoclonal antibody, a reagent tube filled with Pax-5 monoclonal antibody and a reagent tube filled with general type CD3 and Pax-5 immunohistochemical antibody and a packing box for separating and centrally packing the reagent tubes. According to the kit and the application method thereof, a bone marrow smear also can be used for carrying out the classification of lymphocyte, the value of bone marrow smear lymphoma diagnosis is greatly improved, and the technology is relatively simple, is easy to popularize and can be used for pathological diagnosis and therapeutic outcome judgment.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to bone marrow smear staining technology, in particular to a bone marrow smear abnormal lymphocyte staining kit and a use method thereof which are jointly marked with CD3 and PAX5 antibodies. Background technique [0002] Malignant lymphoma (hereinafter referred to as lymphoma) is a group of malignant tumors originating from lymph nodes or extranodal tissues and organs. It is divided into Hodgkin's Lymphoma (HD) according to histopathological examination, molecular biological behavior and clinical characteristics. ) and non-Hodgkin lymphoma (NHL). In Western countries, the incidence of lymphoma accounts for the eighth of the whole tumor disease. In my country, it accounts for the 9th of male malignant tumors and the 11th of female malignant tumors respectively. Bone marrow involvement is a common manifestation of lymphoma, so accurate detection of lymphoma with or without bone marrow in...

AI Technical Summary

Problems solved by technology

Bone marrow biopsy can be added with immunohistochemical staining, which has always played a very critical role in lymphoma involving bone marrow. However, due to the complexity of the technique and the high difficulty of diagnosis, it has been difficult to promote. Only some senior doctors in a few tertiary hospitals Pathology can only be carried out

The technique of bone marrow smear is relatively simple, and it has been widely promoted in China. Many second-level hospitals have carried out this project. However, because bone marrow smears have not been able to perform immunohistochemical staining, acute and chronic lymphocytic leukemia can only be separated, but not Separation of T\B types, bone marrow smear diagnosis is less valuable than biopsy