A universal SNP typing probe in Poaceae

A Poaceae, typing probe technology, applied in the field of molecular biology, can solve the problems of difficulty, limit the application of the Taqman probe method, and high cost of synthesizing probes, and achieve the effect of reducing the experimental cost.
CN104946748BActive Publication Date: 2018-10-19CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
Publication Date
2018-10-19

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Abstract

The invention relates to a universal SNP typing probe in gramineous plants. The probe is composed of two pairs of reverse complement oligonucleotide sequences, and a fluorescence reporter group and a fluorescence quenching group are respectively positioned in reverse complement oligonucleotide chains. A present Taqman probe method is improved to design the SNP typing universal Taqman probe, so the experiment cost is reduced, and a Taqman probe SNP typing method can be widely applied in SNP detection of the gramineous plants.
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Description

technical field

[0001] The invention relates to the fields of molecular biology and nucleic acid detection, and specifically designs a general Taqman probe for SNP typing of Gramineae plants and its application in SNP site typing of Gramineae plants. Background technique

[0002] Poaceae is the most economically valuable category of angiosperms, with more than 650 genera and more than 10,000 species, of which there are more than 200 genera and more than 1,200 species in my country. Poaceae plants are the main source of human food and livestock feed, have high ecological and ornamental value, and are also important raw materials for industry.

[0003] Take wheat as an example. Wheat is the second largest grain crop in my country. In 2013, my country's wheat output reached 121.93 million tons. The steady growth of wheat production and continuous improvement of quality are of great significance to ensure national food security. Wheat varieties with high yield, good quality an...

Claims

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