A visual and rapid method for the detection of trace uranyl ions in aqueous environments
A technology for detecting water and trace uranium, which is applied in color/spectral characteristic measurement, material analysis by observing the influence on chemical indicators, and analysis by making materials react chemically, etc. Detection, low anti-matrix interference ability, poor selectivity and other problems, to achieve good anti-matrix interference ability, strong anti-matrix interference ability, and fast detection speed
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Embodiment 1
[0028] (1) Preparation of DNAzyme functionalized magnetic beads: Take 2.0 μL of substrate strand DNA (sequence: 5'-biotin-ATATAT TGT CCG TGC TAG AAG GAA CTC ACT AT rA GGA AGA GAT GGA CGTG-3 ') and 2.0 μL enzyme chain DNA with a concentration of 0.36 μM (sequence: 5'-CAC GTC CAT CTC TGC AGT CGGGTA GTT AAA CCG ACC TTC AGA CAT AGT GAG T-3') were mixed in 46 μL of MES hybridization buffer (50mM MES, 300mM NaNO 3 , pH=5.5), heated to 85 ℃ for 2 min, then cooled to room temperature at a rate of 1 ℃ / min to obtain a double-stranded DNAzyme solution; then, 10 μL of magnetic bead dispersion with a concentration of 1 mg / mL (surface Modified Streptavidin; diameter: about 1 μm), the mixed solution was shaken at 37 °C for 30 min to evenly immobilize the DNAzyme on the surface of the magnetic beads. Use a magnet to separate the magnetic beads immobilized with DNAzyme double strands, and wash with 100 μL MES hybridization buffer for three times, then disperse the magnetic beads immobilized w...
Embodiment 2
[0033] Take 10 μL of Minjiang water sample filtered through a 0.22 μm filter membrane, operate according to the method of Example 1, and finally observe the color change, and compare the standard UO obtained in Example 1 2 2+ The color or absorbance of the ionic solution, for the UO in the water sample 2 2+ The ions are semi-quantitatively or quantitatively detected, and the detection results are as shown in Table 1, which are basically the same as the measurement results of ICP-MS, indicating that the method of the present invention is a method for visually and rapidly detecting trace uranyl ions in the water environment, and is suitable for On-site testing.
[0034] Table 1 UO in water samples from the Minjiang River using the established method 2 2+ Ion analysis results and comparison with ICP-MS detection results
[0035]
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