Analysis detection method of fetal intrauterine multiple pollutant exposure levels

Abstract

The present invention provides an analysis detection method of fetal intrauterine multiple pollutant exposure levels. The analysis detection method comprises: 1) taking isolated placenta tissue, and adding a n-hexane/acetone solution to extract to obtain an extraction solution; 2) purifying the extraction solution by using a gel permeation chromatography column, leaching with a n-hexane/dichloromethane solution, adding standard samples of fat, PAHs, PCBs, OCPs and PBDEs, and collecting 80-210 mL of the leaching solution; 3) leaching with a n-hexane/dichloromethane solution, and collecting 0-25 mL of the component; and 4) adding an internal standard substance, and carrying out gas chromatography-mass spectrometry analysis. According to the present invention, the particular biological tissue placenta is selected as the organic pollutant attachment carrier so as to reflect the actual exposure levels of the fetal intrauterine multiple pollutants; and the extraction rate of the method on the organic pollutants can achieve 93, the experimental parameters of the sample purification step are accurately set, and the multiple persistent organic pollutants in the human body placenta tissue can be simultaneously detected.

Description

technical field [0001] The invention belongs to the field of analysis, and in particular relates to a method for detecting intrauterine pollutants by combining gas chromatography and mass spectrometry. Background technique [0002] Persistent organic pollutants (POPs) are a class of pollutants that are difficult to degrade in the environment and can be enriched through the food chain and transported over long distances. [1] . POPs have a variety of toxic effects on the human body, including harm to the immune system, endocrine disruption, reproductive and developmental toxicity, and "three-induced" effects, etc. ( [2]，[3]，[4]，[5] . In view of its serious ecological and health effects, more than 100 countries around the world signed the "Stockholm Convention on Persistent Organic Pollutants" (hereinafter referred to as the "Stockholm Convention") in 2001, which stipulates 12 priority control persistent organic pollutants In 2009, nine types of pollutants, including the fla...

AI Technical Summary

Problems solved by technology

[0004] So far, international research on POPs in human placenta is very limited. On the one hand, it is more difficult to collect human placental samples than environmental samples; on the other hand, the POPs content in human placental tissue is very low, and The analysis of placental tissue requires a complicated sample pretreatment process, which requires high experimental conditions and experimental skills

Although some countries in the world have carried out research on POPs in human placenta, the analysis methods used are all based on the method of analyzing environmental samples or slightly improved, which not only consumes a lot of time, but also has certain gaps in the experimental process. Insufficient (such as low recovery rate, incomplete purification, etc.), directly affects the extraction effect of target substances and the accuracy of instrumental analysis

At present, there is no international standard method for the trace analysis of POPs in human placental tissue

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