A method for evaluating the safety of the aquatic environment of transgenic insect-resistant rice

A genetically modified and safe technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of less research on the impact of soil organisms and aquatic organisms, simplify reagents and utensils, reduce costs, and improve detection accuracy Effect

Active Publication Date: 2017-10-24
HUAZHONG AGRI UNIV
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Problems solved by technology

[0011] From the above analysis, it can be seen that the environmental safety assessment of transgenic insect-resistant rice only emphasizes the impact on the above-ground ecosystem, and there are few studies on the impact on soil organisms and aquatic organisms.

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  • A method for evaluating the safety of the aquatic environment of transgenic insect-resistant rice
  • A method for evaluating the safety of the aquatic environment of transgenic insect-resistant rice
  • A method for evaluating the safety of the aquatic environment of transgenic insect-resistant rice

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Embodiment Construction

[0048] The technical solutions of the present invention will be described below in conjunction with the accompanying drawings and embodiments.

[0049] The invention provides a method for evaluating the safety of the aquatic environment of transgenic insect-resistant rice, comprising the following processes:

[0050] (1) Layout and management of transgenic rice fields and non-transgenic rice fields, including the following steps:

[0051] 1) Select 5 fields with flat terrain and convenient water source (for repeated evaluation of environmental safety), with an area of ​​400-600m 2 , the aspect ratio ranges from 2:1 to 1:1, and there is no domestic sewage pollution around the fields and water sources;

[0052] 2) the fields in step 1) are divided into sub-districts, and impermeable field ridges and hardened ditches are built around the fields; in order to prevent rainstorm flooding, a good drainage ditch is built around the fields; the specific scheme for dividing the fields i...

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Abstract

The invention provides a method for evaluating the safety of aquatic environment for genetically modified insect resistant rice. The method comprises the following steps of: arranging and managing genetically modified paddies and non-genetically modified paddies; determining foreign protein in water layers and silt of the paddies, which comprises pretreating samples and extracting and determining the foreign protein; separating plankton in the water layers of the paddies, which comprises collecting water samples and precipitating and separating the plankton; determining the kind and the abundance of the plankton in the water layers of the paddies, which comprises identifying living bodies and counting the number; and planting the genetically modified insect resistant rice and evaluating the safety of the plankton, performing statistical analysis on the kind, the number and the occurrence period of the plankton by using analysis software so as to obtain parameters of a diversity index, a uniformity index and the like, and comparing the parameters with those of conventional paddies so as to evaluate the safety of the aquatic environment for the genetically modified 53 insect resistant rice. The method disclosed by the invention has practical application value for systematically assessing the safety of the aquatic environment for the genetically modified insect resistant rice.

Description

technical field [0001] The invention relates to the technical field of ecological risk assessment of transgenic crops, in particular to a method for evaluating the safety of the aquatic environment of transgenic insect-resistant rice. Background technique [0002] Rice is one of the main crops in our country. Enhancing the resistance of rice itself through transgenic breeding method provides an efficient solution for the control of diseases and insect pests. At present, a batch of transgenic rice varieties with high yield, resistance to diseases and insect pests, and herbicide resistance have been successfully bred. Some of the transgenic rice varieties have entered the stage of national biosafety evaluation such as environmental release tests, which provide food security for our country. Technical reserves are provided. However, the cultivation of genetically modified crops may bring a series of safety problems, which has aroused widespread concern and controversy around t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G7/00
CPCA01G7/00
Inventor 王永模刘标
Owner HUAZHONG AGRI UNIV
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