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A kind of cell culture dish which can prevent cell pollution and its application

A cell culture and cell technology, applied in the direction of tissue cell/virus culture device, special-purpose bioreactor/fermenter, sterilization method, etc., can solve the problems of extended experiment time, cell contamination, waste of experiment funds, etc. , to achieve the effect of improving experimental efficiency, eliminating pollution, and reducing the probability of pollution

Inactive Publication Date: 2017-11-07
HUNAN UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the defects in the prior art that cell contamination is easily caused in the process of cell liquid exchange, resulting in prolongation of experiment time and waste of experiment funds, the present invention provides a cell culture dish that can prevent cell contamination

Method used

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  • A kind of cell culture dish which can prevent cell pollution and its application
  • A kind of cell culture dish which can prevent cell pollution and its application
  • A kind of cell culture dish which can prevent cell pollution and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] This embodiment relates to a cell culture dish that can prevent cell contamination, its front view is as follows figure 1 , top view as figure 2 :

[0039] It includes a cell holding dish 1 , an antibacterial liquid replacement cover 4 covering the cell holding dish, and a pressure assembly sealingly connected with the antibacterial liquid exchange cover.

[0040] The cell holding dish is a hollow cylinder with an open top, and six ventilation columns 3 are arranged on the upper end of the side wall 2 of the hollow cylinder for supporting the antibacterial liquid replacement cover 4 located above the cell carrying dish .

[0041] The antibacterial liquid change cover 4 is a hollow cylinder with an open bottom, and the inner diameter of the antibacterial liquid change cover is larger than the outer diameter of the cell holding dish 1; Groove 9, the liquid-changing groove 9 is a small cylinder sunken inward, the bottom surface of the small cylinder is the antibacteria...

Embodiment 2

[0045] This embodiment relates to a cell culture dish that can prevent cell contamination. Compared with Embodiment 1, the difference is that

[0046] The bacteria-repelling net is a filter membrane with a pore size of 0.1 μm.

[0047]The upper edge of the liquid replacement groove has a downwardly recessed inner edge 11 for supporting the pressure component, and four inwardly recessed card slots 10 are provided on the inner edge. (Such as image 3 shown)

[0048] The bottom of the piston cylinder is provided with an inwardly protruding sealing groove 14, the width of the sealing groove is the same as the width of the inner edge 11 of the liquid changing groove, and a sealing ring 12 is arranged in the sealing groove, and the sealing groove The lower part is provided with 4 locking teeth 13, and the locking teeth coincide with the locking grooves. (Its front view is as Figure 4 As shown, its three-dimensional structure diagram is shown in Figure 5 shown)

[0049] The t...

Embodiment 3

[0052] This embodiment relates to the application of the cell dish that can prevent cell contamination according to the present invention in cell culture, which specifically includes the following steps:

[0053] 1) Inoculate cells in the cell-carrying dish and add culture medium, cover the antibacterial liquid replacement cover, connect a pressure component to the antibacterial liquid replacement cover, and place it in an incubator for cultivation.

[0054] 2) After culturing until the liquid needs to be changed, take out the cell culture dish, pull the pressure rod under aseptic conditions, suck the culture liquid in the cell holding dish to the liquid exchange groove, and remove the pressure rod. Assemblies, suck out the culture fluid in the fluid changing groove.

[0055] 3) Adding fresh culture fluid into the fluid replacement groove, sealingly connecting the pressure assembly with the antibacterial fluid replacement cover, pushing the pressure rod, and hydraulically inje...

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Abstract

The invention relates to a cell culture dish capable of preventing cell contamination. The cell culture dish comprises a cell bearing dish, an antibacterial liquid change cover covering the cell bearing dish, and a pressure assembly connected with the antibacterial liquid change cover in a sealed mode. A culture dish cover does not need to be opened in the cell liquid change process, liquid change can be directly achieved through suction of the pressure assembly, the probability that cells are contaminated is greatly lowered, if a nutrient solution on the upper layer of the antibacterial liquid change cover is contaminated due to various reasons of cells, rapid and effectively rescue processing can be conducted through the cell culture dish, the experiment cost is saved, and the culture time is shortened.

Description

technical field [0001] The invention belongs to the field of cell culture, in particular to a cell culture dish for preventing cell pollution. Background technique [0002] With the continuous update and improvement of science and technology, as well as the continuous research of cell biology and molecular biology, people's exploration of science extends from the macrocosm to the microcosm. With the advent of optical microscopes and electron microscopes, people try to explore the unknown world from the level of cells and molecules. However, bacterial contamination in the cell culture process has become an unavoidable problem. With the continuous improvement of scientific research and technology, people have adopted a series of means to reduce the probability of cell contamination. Even so, the contamination problem in the cell culture process has not been solved Effective solution, which greatly prolongs the experimental period. Especially when cultivating more precious ce...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M3/00C12M1/22C12M1/12
CPCC12M23/10C12M23/38C12M29/14C12M37/02
Inventor 张国民刘慧萍刘平安王灿李玲李迎秋鲍婷婷曾柳庭李启正林政桦
Owner HUNAN UNIV OF CHINESE MEDICINE