Peste des petits ruminants virus antibody detection kit based on ipma

Abstract

The invention provides a peste des petits ruminants virus antibody detection kit based on an immunoperoxidase monolayer assay (IPMA). The kit comprises a 96-well cell culture plate containing intracellular positive antigens and intracellular negative antigens, positive control serum, negative control serum, sample diluents, saline scrubbing solutions, HRP labeled mouse anti-goat McAb, two-component AEC colorimetric solutions, stop solutions, a serum diluting plate and an operating instruction. The intracellular positive antigens are BHK-gSLAM cells infected by a peste des petits ruminant virus rPPRV / GFP, the intracellular negative antigens are BHK-gSLAM cells not infected by the peste des petits ruminant virus, the positive control serum is immune positive serum obtained through blood sampling and separating after a goat is immunized by peste des petits ruminants virus vaccine strains PPRV N75 / 1, and the neutralizing titer is above 1:160; the negative control serum is negative serum obtained through direct blood sampling and separating from a non-immunized peste des petits ruminants negative goat.

Description

technical field [0001] The invention provides a detection kit of Peste des petits ruminants virus antibody based on immunoperoxidase cell monolayer assay (immunoperoxidasemonolayer assay, IPMA), and the kit can detect whether there are small ruminants in the body of small ruminants with high specificity and high sensitivity. Peste des petits ruminants virus (PPRV) antibody, so as to achieve the purpose of vaccine antibody detection or disease diagnosis. Background technique [0002] Peste des petits ruminants virus (PPRV) infection is a severe and contagious infectious disease caused by peste des petits ruminants virus (PPRV), which can cause significant economic losses. The disease is a class A severe infectious disease stipulated by FAO / OIE, and a class I animal epidemic disease stipulated by my country. [0003] Its pathogen is a member of the genus Morbillivirus in the Paramyxoviridae family, and its genome is a single-stranded negative-sense RNA that is not segmented. ...

AI Technical Summary

Problems solved by technology

[0008] Although VNT is a sensitive and specific detection method, it also has some disadvantages, such as (1) time-consuming, generally 10-12 days to obtain the results

(2) Strict conditions, involving cell culture and aseptic treatment of test samples, and experience in judging the results, etc. In developing countries, especially at the grassroots level, there is no condition to carry out

(3) Laborious, heavy workload and labor intensity, not suitable for the detection of a large number of samples

At present, although there are commercialized ELISA kits available in the reference laboratories of Peste des petits ruminants in Britain and France, there are still problems of insufficient sensitivity and specificity, which cannot replace the gold standard VNT method, and the use price is relatively expensive

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