A method for enzymatically extracting polysaccharides from Echinacea purpurea

An extraction method and technology of Echinacea, applied in allergic diseases, drug combination, fermentation and other directions, can solve the problems of low extraction rate, long extraction time of Echinacea polysaccharide, etc., and achieve the effects of high extraction rate, short extraction time and good water solubility

Inactive Publication Date: 2018-07-06
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problems of long extraction time and low extraction rate of Echinacea purpurea polysaccharides, and at the same time obtain better active ingredients, the present invention provides a method for enzymatically extracting echinacea purpurea polysaccharides

Method used

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  • A method for enzymatically extracting polysaccharides from Echinacea purpurea
  • A method for enzymatically extracting polysaccharides from Echinacea purpurea
  • A method for enzymatically extracting polysaccharides from Echinacea purpurea

Examples

Experimental program
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Effect test

Embodiment 1

[0030] The extraction method includes the following steps:

[0031] ① Medicinal material pretreatment: crush the aboveground part of Echinacea purpurea through a 60-mesh sieve; add a degreasing solvent, after degreasing, evaporate petroleum ether to dryness at 60°C, and use the treated medicinal material for later use;

[0032] ②Extraction: Weigh 100g of the medicinal material obtained in ①, add 1500g of water, adjust the pH to 3, preheat to 50°C, add 5g of pectinase, enzymatic hydrolysis time 60min, inactivate enzyme at 80°C for 10min, continue at 80°C Stir and keep warm for 1-h;

[0033] ③ Concentration: centrifuge, and concentrate the supernatant to a concentrated solution with a crude drug amount of 1:0.9 by rotary steaming and reducing pressure;

[0034] ④ Alcohol precipitation: add 3 times of absolute ethanol to the concentrated solution, and let it stand at 4°C for 24 hours;

[0035] ⑤ Washing: Suction filter the alcohol precipitation solution obtained in ④, and wash ...

Embodiment 2

[0038] The extraction method includes the following steps:

[0039] ① Medicinal material pretreatment: crush the aboveground part of Echinacea purpurea through a 40-mesh sieve; add petroleum ether, after degreasing, evaporate the petroleum ether to dryness at 60°C, and use the processed medicinal material for later use;

[0040] ②Extraction: Weigh 100g of the medicinal material obtained in ①, add 1500g of water, adjust the pH to 2, preheat to 40°C, add 4g of pectinase, enzymatic hydrolysis time 50min, inactivate the enzyme at 80°C for 10min, continue at 80°C Stir and keep warm for 1h;

[0041] ③ Concentration: centrifuge, and concentrate the supernatant to a concentrated solution with a crude drug amount of 1:0.9 by rotary steaming and reducing pressure;

[0042] ④ Alcohol precipitation: add 3 times of absolute ethanol to the concentrated solution, and let it stand at 0°C for 24 hours;

[0043] ⑤ Washing: Suction filter the alcohol precipitation solution obtained in ④, and w...

Embodiment 3

[0046] The extraction method includes the following steps:

[0047] ① Medicinal material pretreatment: crush the aboveground part of Echinacea purpurea through a 60-mesh sieve; add petroleum ether degreasing solvent, after degreasing, evaporate the petroleum ether to dryness at 60°C, and use the processed medicinal material for later use;

[0048] ②Extraction: Weigh 100g of the medicinal material obtained in ①, add 2500g of water, adjust the pH to 4, preheat to 60°C, add 6g of pectinase, enzymatic hydrolysis time 70min, inactivate the enzyme at 100°C for 20min, continue at 100°C Stir and keep warm for 2h;

[0049] ③ Concentration: Centrifuge, and concentrate the supernatant to a concentrated solution with a crude drug amount of 1:1.5 by rotary steaming and reducing pressure;

[0050] ④ Alcohol precipitation: Add 4 times of absolute ethanol to the concentrated solution, and let it stand at 4°C for 48 hours;

[0051] ⑤ Washing: Suction filter the alcohol precipitation solution...

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Abstract

The invention discloses a method for extracting polysaccharides from Echinacea purpurea with enzymes. The above-ground part of Echinacea purpurea is crushed into a suitable size, degreased, extracted, enzymatically hydrolyzed by adding an enzyme preparation, deactivated, continued to extract at high temperature, centrifuged, and the supernatant is subjected to rotary evaporation and concentration. sink, and the precipitate was washed successively with absolute ethanol, acetone, ether, redissolved, and sprayed dry to obtain echinacea purpurea polysaccharide. The beneficial effects of the present invention are: the preparation method has the advantages of mild reaction conditions, short extraction time, high extraction rate, high reproducibility and stability, and the prepared Echinacea purpurea polysaccharide has better solubility, uniform appearance and good water solubility, And obtain better active components, have better immune activity.

Description

technical field [0001] The invention relates to the field of polysaccharide extraction, in particular to a method for enzymatically extracting echinacea purpurea polysaccharides. Background technique [0002] Echinacea (Echinacea) is a world-renowned immune herb, native to North America and southern Canada. It is a kind of plant of the genus Echinacea, and this genus has 8 species and several varieties. At present, it mainly contains 3 varieties as medicine development: Echinacea purpurea (Echinacea purpurea, commonly known as Echinacea purpurea), narrow Leaf Echinacea (Echinacea augustifolia) and White Echinacea (Echinacen pallida). Studies have shown that Echinacea purpurea contains a variety of active ingredients, such as caffeic acid derivatives, polysaccharides and glycoproteins, alkamides, alkaloids, polyacetylenes, flavonoids, volatile oils, sterols, etc., and its active ingredients have become current research hotspots. [0003] Echinacea has been called a "natural...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/04C12P19/14A61P37/04
Inventor 郝智慧杨芬芳黄婷婷赵莉
Owner QINGDAO AGRI UNIV
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