Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

GC/MS metabonomics analysis method based on osteoblast cell tissue

An osteoblast and metabolomics technology, which is applied in the field of analyzing metabolites in normal osteoblasts and hydrogen peroxide-induced osteoblasts, can solve the problem that the principles and methods for the establishment of diagnostic criteria for osteoporosis are not uniform and imperfect. Osteoporosis diagnosis methods, etc.

Inactive Publication Date: 2015-10-28
NANJING UNIV OF TECH
View PDF4 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current diagnostic criteria for osteoporosis are not unified in terms of principles and methods, so there is no perfect and definitive diagnostic method for osteoporosis.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • GC/MS metabonomics analysis method based on osteoblast cell tissue
  • GC/MS metabonomics analysis method based on osteoblast cell tissue
  • GC/MS metabonomics analysis method based on osteoblast cell tissue

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0025] Further describe the present invention below in conjunction with example

[0026] 1. Data information and analysis content

[0027] 1.1 Basic information of samples

[0028] 6 groups of normal osteoblasts (control group), 6 groups of osteoblasts intervened with hydrogen peroxide for 24 hours (disease group), 12 groups in total.

[0029] 1.2 Sample preparation

[0030] h 2 o 2For intervening osteoblasts, as well as normal cultured osteoblasts, suck out the medium, wash with normal saline, and break with a cell disruptor. Take the crushed liquid and add internal standard. Vortex and shake for 5 minutes, freeze and centrifuge, take the supernatant and dry it completely with a rotary evaporator. Add methoxyaminopyridine to the evaporated sample, vortex and shake, and incubate at 37°C for 16h, add MSTFA+TMS, vortex shake for 5min, and incubate at 37°C for 1h. After adding the external standard, vortex and shake for 5 minutes, refrigerate and centrifuge for 10 minutes,...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a GC / MS metabonomics analysis method based on osteoblast cell tissue. The method includes the steps of collecting normal osteoblast and osteoblast samples interfered with hydrogen peroxide, conducting gas chromatography separation after processing is conducted, conducting detection analysis through mass spectra, extracting original data of instruments after GC / MS detection analysis is conducted to obtain data which are low in signal to noise ratio and capable of being used for statistic analysis, analyzing the data through principal component analysis and partial least squares analysis to obtain difference metabolin between two sets, and primarily authenticating the metabolin. By means of the method, the variation situations of metabolite of the normal osteoblast and hydrogen peroxide molding cells can be totally, comprehensively and effectively embodied, and the favorable technical support is provided for early diagnosis and prognosis of osteoporosis.

Description

technical field [0001] The present invention relates to a metabolomic analysis method based on osteoblast cell tissue GC / MS, in particular to a method for analyzing metabolites in normal osteoblasts and hydrogen peroxide-induced osteoblasts by using GC / MS full-scan mode analysis method method. Background technique [0002] Osteoporosis is a disease that seriously endangers human health and even life. Statistics from the World Health Organization show that the incidence of osteoporosis has jumped to the seventh place among the common frequently-occurring diseases in the world. The total number of patients in the world exceeds 200 million, and the annual treatment and hospitalization costs are as high as 25 billion US dollars. In my country, according to the epidemiological survey report, the incidence rate of the crowd of 50-60 years old is 21%, the incidence rate of the crowd of 60-70 years old is 58%, and the incidence rate of the crowd of 70-80 years old is 100%. Especia...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N30/88
Inventor 张琪马博唐博文祁欢欢李静
Owner NANJING UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com