Organic fertilizer prepared by using stems and leaves of fruit of towel gourd and balsam pear
A technology for organic fertilizers and bitter gourd stems and leaves, applied in the field of organic fertilizers and fertilizers, can solve the problems of no prevention and treatment of rape sclerotinia, and the preparation of organic fertilizers from loofah stems and bitter gourd stems and leaves.
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Embodiment 1
[0048] The preparation method of organic fertilizer is as follows:
[0049] (1) Bacterial strain expansion culture: get Trichoderma koningeni, Aspergillus niger, inoculate in the liquid PDA culture medium, shake table culture and expand, make containing bacteria liquid;
[0050] (2) Preparation of solid medium: Weigh 1 part of loofah stem and leaf and 1 part of bitter gourd stem and leaf according to the mass, crush them, put them in a 70°C oven for drying, add water and Glucose, mixed well, as a solid medium;
[0051] (3) Solid fermentation: inoculate at a ratio of adding 10 mL of the bacteria-containing solution per 100 g of basic raw materials, and cultivate for 8 days at 28-35° C.;
[0052] (4) Sterilization and drying: Take the material obtained in step (3), sterilize it under high pressure, put it in an oven at 100°C and dry it to obtain the final product.
Embodiment 2
[0054] The preparation method of organic fertilizer is as follows:
[0055] (1) Bacterial strain expansion culture: get Trichoderma koningeni, Aspergillus niger, inoculate in the liquid PDA culture medium, shake table culture and expand, make containing bacteria liquid;
[0056] (2) Preparation of solid medium: Weigh 1 portion of loofah stem and leaf and 1 portion of bitter gourd stem and leaf by weight, crush them, put them in a 90°C oven for drying, add water and Glucose, mixed well, as a solid medium;
[0057] (3) Solid fermentation: inoculate at a ratio of adding 30 mL of the bacteria-containing solution per 100 g of basic raw materials, and cultivate for 12 days at 28-35° C.;
[0058](4) Sterilization and drying: take the material obtained in step (3), sterilize it under high pressure, put it in an oven at 100°C and dry it to obtain the final product.
Embodiment 3
[0060] The preparation method of organic fertilizer is as follows:
[0061] (1) Bacterial strain expansion culture: get Trichoderma koningeni, Aspergillus niger, inoculate in the liquid PDA culture medium, shake table culture and expand, make containing bacteria liquid;
[0062] (2) Preparation of solid medium: Weigh 1 portion of loofah stem and leaf and 1 portion of bitter gourd stem and leaf by weight, crush them, put them in an oven at 80°C to dry, add water and Glucose, mixed well, as a solid medium;
[0063] (3) Solid fermentation: inoculate at the ratio of adding 20 mL of the bacterium-containing liquid per 100 g of basic raw materials, and cultivate for 10 days at 28-35° C.;
[0064] (4) Sterilization and drying: take the material obtained in step (3), sterilize it under high pressure, put it in an oven at 100°C and dry it to obtain the final product.
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