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Fluorescence probe for detecting human serum albumin and preparation method therefor

A technology of serum albumin and fluorescent probes, applied in the direction of fluorescence/phosphorescence, chemical instruments and methods, luminescent materials, etc., can solve the problems of unsatisfactory detection of HSA molecular probes, etc., and achieve a simple process and easy realization Effect

Active Publication Date: 2015-11-04
SHANDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

In the prior art, there is still no satisfactory molecular probe for detecting the concentration of HSA

Method used

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  • Fluorescence probe for detecting human serum albumin and preparation method therefor
  • Fluorescence probe for detecting human serum albumin and preparation method therefor
  • Fluorescence probe for detecting human serum albumin and preparation method therefor

Examples

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Effect test

Embodiment 1

[0030] Preparation of fluorescent probes for detecting human serum albumin:

[0031] (1) Dissolve 1mol of p-amino-N,N-dimethylaniline and 5mol of trans-crotonin in 25mL of toluene, stir and react at room temperature, monitor by TLC, and wait for p-amino-N,N-dimethylaniline After completely participating in the reaction, stop the reaction, extract (mixture of dichloromethane and distilled water) to obtain a crude product, and purify by column chromatography (dichloromethane:methanol=1:1) to obtain product a;

[0032] (2) 0.8mol product a, 1.2mol SeO 2 Add to 25mL dioxane and stir to dissolve, reflux reaction, TLC monitors the reaction, stop the reaction after product a completely participates in the reaction, cool to room temperature, and filter the mixture with diatomaceous earth eluted with dichloromethane, collect The filtrate was evaporated to remove the solvent to obtain a crude product, which was purified by silica gel column chromatography (dichloromethane:methanol=50:1...

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Abstract

The invention relates to a fluorescence probe, in particular to a fluorescence probe for detecting human serum albumin and a preparation method therefor. The molecular structural formula of the fluorescence probe is as shown in the specification. Quinoline fluorogen is used as a reporter group, a fluorescence ratio probe combined with diethyl oxalate has better effect in HSA detection; and the preparation method is simple in technology and easy to realize.

Description

technical field [0001] The invention relates to a fluorescent probe, in particular to a fluorescent probe for detecting human serum albumin and a preparation method thereof. Background technique [0002] Human serum albumin (HSA) is the most abundant protein in human plasma, and its concentration in plasma is as high as 0.7mM. Human serum albumin can reversibly bind many endogenous and exogenous compounds, such as hormones, fatty acids, vitamins, heme, metabolites, and drug molecules, enhance the solubility of these compounds in plasma, and transport them to specific tissues or organs or degrade when they become toxic. The sponge-like function of human serum albumin largely affects the pharmacokinetics of many exogenous drugs. [0003] HSA exhibits pseudoesterase activity and can hydrolyze drugs containing ester bonds, such as aspirin. Lys 199, Lys 402, Lys 519 and Lys 545 constitute the active site of HSA pseudoesterase. [0004] The level of plasma albumin in human bod...

Claims

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Application Information

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IPC IPC(8): C09K11/06C07D215/38G01N21/64
Inventor 陈志伟朱海亮孙娟
Owner SHANDONG UNIV OF TECH
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