Detection and application of gastric carcinogenesis associated molecular marker IncRNA (long non-coding RNA) HOTTIP (HOXA transcript at the distal tip)

A molecular marker, gastric cancer technology, applied in the field of tumor molecular biology, has achieved important promotion and application prospects, far-reaching clinical significance and effective results

Inactive Publication Date: 2015-11-04
王振宁
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  • Detection and application of gastric carcinogenesis associated molecular marker IncRNA (long non-coding RNA) HOTTIP (HOXA transcript at the distal tip)
  • Detection and application of gastric carcinogenesis associated molecular marker IncRNA (long non-coding RNA) HOTTIP (HOXA transcript at the distal tip)
  • Detection and application of gastric carcinogenesis associated molecular marker IncRNA (long non-coding RNA) HOTTIP (HOXA transcript at the distal tip)

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Embodiment 1

[0048] Real-time fluorescence quantitative PCR preliminarily verified the differential expression of lncRNA HOTTIP in gastric cancer tissues and adjacent normal tissues.

[0049] Collect gastric cancer and paracancerous tissues to be tested into cryopreservation tubes and store at -80°C for later use.

[0050] 1. Extraction of total RNA in tissues: Extract total RNA from gastric cancer tissues or normal paracancerous tissues according to the reagents and steps required by Life Technologies' reagent Trizol (Catalog No.: 15596-026). The main operation steps are as follows:

[0051] (1) Put 50-100 mg of tissue into an RNase / DNase-free centrifuge tube (Axygen, 1.5 ml), add 1 ml of Trizol, cut it into pieces with scissors and homogenize it quickly, then put it at room temperature for 5 minutes;

[0052] (2) Add 200ul / ml Trizol in chloroform to the centrifuge tube, shake it by hand for 15s, and place it at room temperature for 5min;

[0053] (3) Centrifuge at 12000g for 15min at 4...

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Abstract

The invention relates to a gastric cancer associated molecular marker IncRNA (long non-coding RNA) HOTTIP (HOXA transcript at the distal tip), an application of the molecular marker in auxiliary diagnosis of gastric cancer as well as a gastric carcinogenesis predicting kit. According to a sequence of the IncRNA, specific real-time fluorescent quantitative PCR (polymerase chain reaction) primers are designed and synthesized, and a reagent for auxiliary diagnosis of gastric cancer is prepared. Real-time fluorescent quantitative PCR proves that HOTTIP has differential expression between gastric cancer tissue and normal tissue beside the gastric cancer tissue and has down-regulated expression in the gastric cancer tissue. The gastric cancer associated molecular marker IncRNA HOTTIP can be used for evaluation and diagnosis of gastric carcinogenesis, facilitates screening of individuals having high risks of the gastric cancer and guides making of reasonable monitoring, following, processing and treating schemes clinically.

Description

Technical field: [0001] The invention belongs to the field of tumor molecular biology. Specifically, the invention relates to a tumor molecular marker lncRNA HOTTIP related to the occurrence of gastric cancer and its auxiliary diagnostic kit. Background technique: [0002] Long non-coding RNA (lncRNA) is a class of RNA molecules longer than 200 bp in length, lacking a specific open reading frame, and not having protein-coding functions. In recent years, studies have shown that lncRNA is involved in epigenetic modification and plays an important role in the regulation of gene expression. At present, lncRNA has played an important role in the clinical diagnosis, treatment and prognosis evaluation of various diseases such as tumors, blood diseases, and cardiovascular diseases. . [0003] China is a large country with gastric cancer. Gastric cancer in my country has the characteristics of high morbidity and mortality, low proportion of early gastric cancer, and advanced cases a...

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6886C12Q2600/158C12Q2600/178
Inventor 王振宁宋永喜杨宇翀马斌高鹏王嘉俊赵俊华王俊
Owner 王振宁
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