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Node using method of high-concentration IBA and glycerin gel

A glycerin gel, high-concentration technology, applied in the field of plant biology, can solve the problems of branch loss, high cost, and many technical steps, and achieve the effect of reducing water evaporation and easy operation

Inactive Publication Date: 2015-11-18
钱夕华
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, branches that are difficult to differentiate during in vitro propagation or rooting are extremely prone to death due to long-term loss of water or nutrients
However, the existing method of root-promoting and bud-inducing flowering has disadvantages such as many technical steps, high cost, instability, etc., and the effect in production is not satisfactory.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0013] A method for using high-concentration IBA and glycerin gel at nodes, characterized in that the method includes preparation of hormone mother solution, preparation of working solution, preparation of hormone gel, cutting, dripping at axillary buds, and the specific operation steps are as follows:

[0014] ① Hormone mother solution preparation: Accurately weigh 0.1g of IBA and dissolve it with a little 95% ethanol, then dilute to 100mL with distilled water, which is 1mg / mL IBA mother solution;

[0015] ②Preparation of working solution: Dilute the above mother solution according to the volume ratio of 1:2 into a high-concentration hormone working solution with a concentration of 500mg / L, and add 10ml of glycerin to 90ml of hormone working solution at the same time;

[0016] ③ Hormone gel preparation: Accurately weigh 1g of sodium carboxymethylcellulose and 8g of sodium alginate, and dissolve them in 100mL of hormone and glycerin working solution with a concentration of 500m...

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PUM

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Abstract

The invention discloses a node using method of high-concentration IBA and glycerin gel, and belongs to the technical field of plant biology. The method includes the steps that IBA mother liquid with the concentration of 1 mg / mL is prepared; the mother liquid is diluted into high-concentration IBA working liquid with the concentration of 500 mg / L; the working liquid is prepared into high-concentration IBA gel and glycerin gel confining liquid containing sodium carboxymethylcellulose with the mass concentration of 1% and sodium alginate with the mass concentration of 8%; branches are inserted into a culture medium; the gel confining liquid is dripped to axillary buds of the healthy and stocky branches.

Description

technical field [0001] The invention discloses a node-site application method of high-concentration IBA and glycerin gel, which belongs to the field of plant biotechnology. Background technique [0002] Cutting propagation is one of the commonly used methods for asexual propagation of plants, especially trees. This method has many advantages such as low cost, quick effect, short cycle, abundant sources of branches used, and ability to maintain the excellent traits of the parents. Different types of hormones are often used in cutting propagation, such as IBA, IBA, NAA, 2,4-D, GA 3 , 6-BA, KT, TDZ, TIBA, Ethephon, B 9 , PP333, MH, etc. The addition of the above-mentioned hormones will enable plants to take early or delayed rooting, flowering, and fruit setting under in vitro or in vitro conditions, increase the number of roots, induce axillary bud germination, and increase fruit setting rates. important use. However, branches that are difficult to differentiate during in vi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N25/04A01N43/38A01P21/00A01G1/00A01G7/06
Inventor 钱夕华
Owner 钱夕华