Method for producing goat-anti-human cystatin C protein antiserum
A cystatin, antiserum technology, applied in the direction of anti-peptide structure protease inhibitor immunoglobulin, serum immunoglobulin, etc., can solve the problems of high antigen dosage, low level, slow antibody production, etc. The effect of small dosage, simple process and high antibody level
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Embodiment 1
[0030] Choose 5 adult healthy soil goats, carry out the following steps:
[0031] (1) Material preparation: select human cystatin C protein antigen, sterile PBS, complete Freund's adjuvant and incomplete Freund's adjuvant for standby;
[0032] (2) The first injection: Dilute 1.25 mg of human cystatin C protein antigen to 10 ml with sterile PBS, then use 10 ml of complete Freund's adjuvant to make immunogen emulsion, and inject it into the sheep. The injection site is the left and right feet. 2 points on the bottom, 4 points under the skin on the left and right groins, 2 points under the skin on both sides of the neck, and 4 points under the skin on the back, a total of 12 injection points. The injection dose for each sheep is 4ml containing 0.25mg of antigen, and the average injection is;
[0033] (3) Second injection: 15 days after the first injection, the second injection is made by diluting 2.5 mg of human cystatin C protein antigen to 10 ml with sterile PBS, and then using...
Embodiment 2
[0044] Choose 5 adult healthy soil goats, carry out the following steps:
[0045] (1) Material preparation: select human cystatin C protein antigen, sterile PBS, complete Freund's adjuvant and incomplete Freund's adjuvant for standby;
[0046](2) The first injection: Dilute 1.25 mg of human cystatin C protein antigen to 10 ml with sterile PBS, then use 10 ml of complete Freund's adjuvant to make immunogen emulsion, and inject it into the sheep. The injection site is the left and right feet. 2 points on the bottom, 4 points under the skin on the left and right groins, 2 points under the skin on both sides of the neck, and 4 points under the skin on the back, a total of 12 injection points. The injection dose for each sheep is 4ml containing 0.25mg of antigen, and the average injection is;
[0047] (3) Second injection: 15 days after the first injection, the second injection is made by diluting 2.5 mg of human cystatin C protein antigen to 10 ml with sterile PBS, and then using ...
Embodiment 3
[0058] Choose 5 adult healthy soil goats, carry out the following steps:
[0059] (1) Material preparation: select human cystatin C protein antigen, sterile PBS, complete Freund's adjuvant and incomplete Freund's adjuvant for standby;
[0060] (2) The first injection: Dilute 1.25 mg of human cystatin C protein antigen to 10 ml with sterile PBS, then use 10 ml of complete Freund's adjuvant to make immunogen emulsion, and inject it into the sheep. The injection site is the left and right feet. 2 points on the bottom, 4 points under the skin on the left and right groins, 2 points under the skin on both sides of the neck, and 4 points under the skin on the back, a total of 12 injection points. The injection dose for each sheep is 4ml containing 0.25mg of antigen, and the average injection is;
[0061] (3) Second injection: 15 days after the first injection, the second injection is made by diluting 2.5 mg of human cystatin C protein antigen to 10 ml with sterile PBS, and then using...
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