Method for producing goat-anti-human cystatin C protein antiserum

A cystatin, antiserum technology, applied in the direction of anti-peptide structure protease inhibitor immunoglobulin, serum immunoglobulin, etc., can solve the problems of high antigen dosage, low level, slow antibody production, etc. The effect of small dosage, simple process and high antibody level

Inactive Publication Date: 2015-11-18
海奥斯生物科技镇江有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the current production methods can produce a certain amount of antibodies, the antibody production speed is slow, the level is low, and the amount of antigen is high

Method used

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  • Method for producing goat-anti-human cystatin C protein antiserum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Choose 5 adult healthy soil goats, carry out the following steps:

[0031] (1) Material preparation: select human cystatin C protein antigen, sterile PBS, complete Freund's adjuvant and incomplete Freund's adjuvant for standby;

[0032] (2) The first injection: Dilute 1.25 mg of human cystatin C protein antigen to 10 ml with sterile PBS, then use 10 ml of complete Freund's adjuvant to make immunogen emulsion, and inject it into the sheep. The injection site is the left and right feet. 2 points on the bottom, 4 points under the skin on the left and right groins, 2 points under the skin on both sides of the neck, and 4 points under the skin on the back, a total of 12 injection points. The injection dose for each sheep is 4ml containing 0.25mg of antigen, and the average injection is;

[0033] (3) Second injection: 15 days after the first injection, the second injection is made by diluting 2.5 mg of human cystatin C protein antigen to 10 ml with sterile PBS, and then using...

Embodiment 2

[0044] Choose 5 adult healthy soil goats, carry out the following steps:

[0045] (1) Material preparation: select human cystatin C protein antigen, sterile PBS, complete Freund's adjuvant and incomplete Freund's adjuvant for standby;

[0046](2) The first injection: Dilute 1.25 mg of human cystatin C protein antigen to 10 ml with sterile PBS, then use 10 ml of complete Freund's adjuvant to make immunogen emulsion, and inject it into the sheep. The injection site is the left and right feet. 2 points on the bottom, 4 points under the skin on the left and right groins, 2 points under the skin on both sides of the neck, and 4 points under the skin on the back, a total of 12 injection points. The injection dose for each sheep is 4ml containing 0.25mg of antigen, and the average injection is;

[0047] (3) Second injection: 15 days after the first injection, the second injection is made by diluting 2.5 mg of human cystatin C protein antigen to 10 ml with sterile PBS, and then using ...

Embodiment 3

[0058] Choose 5 adult healthy soil goats, carry out the following steps:

[0059] (1) Material preparation: select human cystatin C protein antigen, sterile PBS, complete Freund's adjuvant and incomplete Freund's adjuvant for standby;

[0060] (2) The first injection: Dilute 1.25 mg of human cystatin C protein antigen to 10 ml with sterile PBS, then use 10 ml of complete Freund's adjuvant to make immunogen emulsion, and inject it into the sheep. The injection site is the left and right feet. 2 points on the bottom, 4 points under the skin on the left and right groins, 2 points under the skin on both sides of the neck, and 4 points under the skin on the back, a total of 12 injection points. The injection dose for each sheep is 4ml containing 0.25mg of antigen, and the average injection is;

[0061] (3) Second injection: 15 days after the first injection, the second injection is made by diluting 2.5 mg of human cystatin C protein antigen to 10 ml with sterile PBS, and then using...

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Abstract

The invention provides a method for producing a goat-anti-human cystatin C protein antiserum; the method includes the following steps: (1) antigen preparation; (2) first injection; (3) second injection; (4) third injection; (5) fourth injection; (6) fifth injection; (7) sixth injection; (8) seventh injection; (9) eighth injection; (10) serum taking; and (11) serum titer detection: taking the antiserum, and detecting the titer. Compared with the prior art, the method for producing the goat-anti-human cystatin C protein antiserum is low in cost and simple in process, and not only can stably produce antibodies, but also can has fast antibody producing speed; and the antibody level is high and the antigen dosage is small.

Description

technical field [0001] The invention relates to a method for producing goat anti-human cystatin C protein antiserum, which belongs to the technical field of antiserum preparation. Background technique [0002] Small-molecule antigens refer to molecular weights between 4KD-10KD, and macromolecular antigens greater than 150KD. Cystatin C (Cys-C) has a smaller molecular weight of 13KD, and its surface area is correspondingly smaller. The probability of contacting immune cells is less, and it binds If it is not firm, its immunogenicity is not strong; the production of antibodies is also related to the molecular structure, the more complex the stronger the immunogenicity, and vice versa; there are many factors that affect the production of high-quality antibodies. [0003] At present, after the human cystatin C protein antigen is emulsified with an adjuvant, animals are immunized according to certain procedures, and anti-human cystatin C protein antibodies can be produced in anim...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/38C07K16/06
Inventor 徐志伟张传明袁金城
Owner 海奥斯生物科技镇江有限公司
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