Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Function and application of angiotensin invertase (ACE3) in treating cardiac hypertrophy

An angiotensin, myocardial hypertrophy technology, applied in the field of gene function and application, to achieve the effect of anti-cardiac fibrosis myocardial hypertrophy, inhibition of myocardial hypertrophy, and protection of cardiac function

Active Publication Date: 2015-12-02
WUHAN UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, studies have reported that ACE3 is involved in the collection of sperm and eggs, but the lack of ACE3 does not affect the combination of sperm and eggs[28]

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Function and application of angiotensin invertase (ACE3) in treating cardiac hypertrophy
  • Function and application of angiotensin invertase (ACE3) in treating cardiac hypertrophy
  • Function and application of angiotensin invertase (ACE3) in treating cardiac hypertrophy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2A

[0087] Example 2 Effect of ACE3 interference (AdshACE3) and overexpression (AdACE3) adenovirus on AngII-stimulated primary cardiomyocyte hypertrophy

[0088] 1. Primary neonatal SD rat cardiomyocyte culture

[0089] (1) Eight newborn Sprague-Dawley suckling mice were disinfected with 75% alcohol below the neck, and the heart was removed with ophthalmic scissors and micro forceps, and placed in a glass plate filled with 10mL DMEM / F12 solution. Take another one and repeat the above process.

[0090] (2) Wash the heart with DMEM / F12 medium, and cut the heart into 1-2mm 3 fragments. Transfer to a serum bottle with a rotor, suck off DMEM / F12, and add trypsin digestion solution. Rotate at 120r / min, digest for 15min, rest for a few seconds, and discard the supernatant.

[0091] (3) Add trypsin digestion solution, the speed is 120r / min, and digest for 15min. Stand still for a few seconds, aspirate the supernatant, terminate the digestion with DMEM / F12 medium with 20% calf serum, ...

Embodiment 3

[0097] Example 3 Myocardial hypertrophy and fibrosis detection and cardiac function detection in myocardial hypertrophy model mice

[0098] 1. Using aortic arch constriction (AB) to establish a mouse model of myocardial hypertrophy

[0099] Wild-type mice (WT), ACE3 knockout mice (ACE3-KO), heart-specific ACE3 transgenic mice (ACE3-TG), and non-transgenic mice aged 8-10 weeks and weighing 23.5-27.5 g were selected. Mice (NTG) were divided into sham operation group (Sham) and AB myocardial hypertrophy model group, namely WTSham group, WTAB group, ACE3-KOSham group, ACE3-KOAB group, ACE3-TGSham group, ACE3-TGAB group, NTGSham group , NTGAB group, 10 mice in each group. The modeling method is the same as in Example 1. The detection of myocardial hypertrophy and fibrosis and the detection of cardiac function were carried out 4 weeks after AB.

[0100] 2. Take materials

[0101] (1) Preliminary work: prepare in advance a urine cup filled with 20mL of 10% formaldehyde by volume,...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a function and an application of angiotensin invertaste (ACE3) in treating cardiac hypertrophy, and belongs to the field of function and application of genes. An interrelation between the expression of ACE3 and cardiac hypertrophy is determined, a research result shows that in a model having the cardiac hypertrophy, the expression of the ACE3 is remarkably decreased compared with that of a normal group; the cardiac hypertrophy, fiberization and heart function deteriorate are remarkably promoted by inhibiting the expression of ACE3, the cardiac hypertrophy and fiberization are remarkably inhibited by promoting the over-expression of the ACE3, and the heart function is protected. Therefore, ACE3 can be used as a target gene to screen a drug for protecting the heart function, resisting the fiberization of the heart and / or preventing, alleviating and / or treating cardiac hypertrophy, to prepare a drug for protecting the heart function, resisting the cardiac hypertrophy and / or preventing, alleviating and / or treating cardiac hypertrophy, and an effective new way is provided for treating the cardiac hypertrophy.

Description

technical field [0001] The invention belongs to the field of gene function and application, and particularly relates to the function and application of angiotensin-converting enzyme 3 (ACE3) in treating myocardial hypertrophy. Background technique [0002] Myocardial hypertrophy is a compensatory response of the myocardium to long-term pressure or increased volume load, which is mainly characterized by increased cardiomyocyte volume and extracellular matrix[1-3]. Although myocardial hypertrophy is initially a compensatory mechanism, long-term persistent pressure or volume loading can cause decompensation, leading to dilated cardiomyopathy, heart failure, and even sudden death[4,5]. Myocardial hypertrophy has become an independent risk factor for cardiovascular diseases such as heart failure, greatly increasing the morbidity and mortality of heart failure, and is more harmful than smoking and hypercholesterolemia [5-7]. Studies have shown that with the occurrence and develop...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/48A61K45/00A61P9/00C12Q1/68
Inventor 李红良张志仁于长江张晓晶
Owner WUHAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com