A Ceratocystis strain and its application in the preparation of valerian, sedative and hypnotic Chinese patent medicines

A technology of horn basidiomycete and valerian, which is applied to endophytic fungi and its application fields, can solve the problems that valerian has not yet been seen, and achieve good industrial application prospects, sedative and hypnotic effects, and good effects

Inactive Publication Date: 2018-07-06
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For those skilled in the art, there are no relevant reports on the industrial production of valerianin using endophytic fungi

Method used

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  • A Ceratocystis strain and its application in the preparation of valerian, sedative and hypnotic Chinese patent medicines
  • A Ceratocystis strain and its application in the preparation of valerian, sedative and hypnotic Chinese patent medicines
  • A Ceratocystis strain and its application in the preparation of valerian, sedative and hypnotic Chinese patent medicines

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Embodiment

[0029] We adopt the method of microbiology, isolate the endophytic fungus that produces valerenin from the healthy valerian plant, then utilize PDA liquid medium to ferment, adopt HPLC method to measure the content of valerenin in the fermented liquid, determine that it is An endophytic fungus producing valerianin was identified through morphological observation and ITS sequence analysis, and its species was determined to be Ceratobacterium XCS-6. The whole isolation and screening , Identify and prepare the specific operation process of valerian as follows.

[0030] 1. Isolation and screening of endophytic fungi producing valerianin

[0031] Valerian explants: Valerian is collected from the mountainous area of ​​Guiyang City, Guizhou Province. It is identified as Valeriana officinale by Professor Jiang Lijuan of Central South University of Forestry and Technology.

[0032] Explant disinfection: the fresh valerian root was rinsed with tap water for 10 minutes, and washed 3 tim...

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Abstract

A strain of Clarobacterium, which is named as Clarobacterium XCS-6, has a preservation number of CGMCC No.9817 in the General Microorganism Center of China Microbiological Culture Collection Management Committee, and the preservation date is October 22, 2014. The sclerotium strain can be applied to prepare valerenin. Specifically, the sclerotium strain is fermented and cultivated by using a liquid medium, oscillating on a shaker, and cultivated at normal temperature for at least 6 days to obtain a valerenin-containing fermented liquid. After firstly applying the clariatium XCS-6 of the present invention to prepare valerianin, the valerianin can also be used to prepare sedative and hypnotic Chinese patent medicines.

Description

technical field [0001] The invention relates to a valerianin-producing endophytic fungus and its application, in particular to a sclerobacter strain and its application. Background technique [0002] As a new microbial resource, plant endophytes have attracted extensive attention from scholars at home and abroad. Discovering new active substances from plant endophytes has become a research hotspot. Studies have shown that endophytic fungi parasitic in medicinal plants can not only promote the production of certain secondary metabolites in host plants, but also produce the same or similar metabolites themselves. Most of the metabolites have obvious anti-tumor, anti-oxidation, antibacterial and other effects, and are good pharmaceutical raw materials. [0003] Valerian (Valeriana officinalis L.) is a plant of the genus Valeriana in the family Patillaceae. It is an important traditional medicinal plant in my country. Its rhizome and root are used as medicine, and it has the ef...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): C12N1/14C12P17/06A61K31/19A61P25/20C12R1/645
Inventor黎继烈胡铁谭云方勤敏王卫贾媛李振梅谷政伟张梦君
OwnerCENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY